dc.creator | Orfanidou, T. | en |
dc.creator | Iliopoulos, D. | en |
dc.creator | Malizos, K. N. | en |
dc.creator | Tsezou, A. | en |
dc.date.accessioned | 2015-11-23T10:41:51Z | |
dc.date.available | 2015-11-23T10:41:51Z | |
dc.date.issued | 2009 | |
dc.identifier | 10.1111/j.1582-4934.2009.00678.x | |
dc.identifier.issn | 1582-1838 | |
dc.identifier.uri | http://hdl.handle.net/11615/31499 | |
dc.description.abstract | Chondrocytes' hypertrophy includes metabolic changes, matrix remodelling, proliferation and apoptosis, characteristics associated with the progression of osteoarthritis. We investigated a possible association among Runt-related transcription factor 2 (RUNX-2), SOX-9 and fibroblast growth factor (FGF)-23 mRNA expressions in articular chondrocytes in order to elucidate their contribution in the osteoarthritic hypertrophic cartilage. SOX-9, FGF-23, RUNX-2 and matrix metalloproteinase (MMP)-13 mRNA expressions were evaluated in osteoarthritic and normal chondrocytes by real-time PCR whereas MMP-13 protein expression by immunofluorescense. RUNX-2, FGF-23 and SOX-9 were down-regulated using small interfering RNA technology and transfection with liposomes. The effect of human recombinant FGF-23 (hrFGF-23) on SOX-9 and RUNX-2 expression was tested in normal chondrocytes. We found higher expression of RUNX-2 and FGF-23 and a decreased expression of SOX-9 mRNA in osteoarthritic chondrocytes compared to normal (P < 0.0001). RUNX-2 down-regulation resulted in reduced MMP-13 expression in osteoarthritic chondrocytes and inhibition of SOX-9 in increased RUNX-2 and MMP-13 mRNA expression in normal chondrocytes, whereas inhibition of FGF-23 resulted in reduced RUNX-2 mRNA expression in osteoarthritic chondrocytes (all P < 0.0001). Silencing of RUNX-2 or FGF-23 did not affect SOX-9 mRNA levels in osteoarthritic chondrocytes. Moreover simultaneous down-regulation of SOX-9 and up-regulation of FGF-23 mRNA expressions in normal chondrocytes resulted in additive up-regulation of RUNX-2 mRNA expression. Treatment of normal chondrocytes with hrFGF-23 resulted in increased RUNX-2 mRNA expression, whereas it had no effect on SOX-9 mRNA expression. We demonstrated convincing associations among RUNX-2, SOX-9 and FGF-23 in relation to MMP-13 expression in osteoarthritic chondrocytes, contributing to a better understanding of the abnormal gene expression and cartilage degeneration processes associated with osteoarthritis. | en |
dc.source | Journal of Cellular and Molecular Medicine | en |
dc.source.uri | <Go to ISI>://WOS:000274179300020 | |
dc.subject | RUNX-2 | en |
dc.subject | SOX-9 | en |
dc.subject | FGF-23 | en |
dc.subject | osteoarthritis | en |
dc.subject | chondrocytes | en |
dc.subject | ADVANCED SECONDARY HYPERPARATHYROIDISM | en |
dc.subject | ENDOCHONDRAL OSSIFICATION | en |
dc.subject | DIFFERENTIATION MARKERS | en |
dc.subject | TRANSCRIPTION FACTOR | en |
dc.subject | SKELETAL DEVELOPMENT | en |
dc.subject | BONE-FORMATION | en |
dc.subject | RETINOIC ACID | en |
dc.subject | RENAL-FAILURE | en |
dc.subject | II COLLAGEN | en |
dc.subject | EXPRESSION | en |
dc.subject | Cell Biology | en |
dc.subject | Medicine, Research & Experimental | en |
dc.title | Involvement of SOX-9 and FGF-23 in RUNX-2 regulation in osteoarthritic chondrocytes | en |
dc.type | journalArticle | en |