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Evaluation of a serum-based PCR assay for the diagnosis of canine monocytic ehrlichiosis
dc.creator | Mylonakis, M. E. | en |
dc.creator | Siarkou, V. I. | en |
dc.creator | Leontides, L. | en |
dc.creator | Bourtzi-Hatzopoulou, E. | en |
dc.creator | Kontos, V. I. | en |
dc.creator | Koutinas, A. F. | en |
dc.date.accessioned | 2015-11-23T10:40:16Z | |
dc.date.available | 2015-11-23T10:40:16Z | |
dc.date.issued | 2009 | |
dc.identifier | 10.1016/j.vetmic.2009.04.015 | |
dc.identifier.issn | 0378-1135 | |
dc.identifier.uri | http://hdl.handle.net/11615/31233 | |
dc.description.abstract | The aim of this study was to estimate the relative diagnostic sensitivity and specificity of a polymerase chain reaction (PCR) assay in the serum of dogs with naturally occurring non-myelosuppressive canine monocytic ehrlichiosis (CME), and to investigate the association between PCR positivity and immunofluorescence antibody (IFA) titres for Ehrlichia canis. Serum samples obtained from 38 dogs with non-myelosuppressive CME and 12 healthy dogs were analyzed retrospectively. Each serum sample was analyzed in triplicate using an E. canis-specific nested PCR assay targeting a 389 bp sequence of the 16S rRNA gene. E. canis DNA was amplified in 24 of 38 (63.1%) affected dogs; all samples from healthy dogs were negative. A high level of agreement was found among the PCR replicates (P < 0.0001). Median IFA titre of the 24 PCR-positive dogs was significantly lower than that of the PCR-negative infected dogs (P = 0.0029), indicating that E. canis DNA may circulate prior to the development of a high antibody titre. Serum-based PCR analysis is suggested for the early diagnosis of CME when whole blood samples are not available. (C) 2009 Elsevier B.V. All rights reserved. | en |
dc.source | Veterinary Microbiology | en |
dc.source.uri | <Go to ISI>://WOS:000270246800025 | |
dc.subject | Serum | en |
dc.subject | Dog | en |
dc.subject | Ehrlichia canis | en |
dc.subject | PCR | en |
dc.subject | 16S rRNA | en |
dc.subject | NESTED PCR | en |
dc.subject | INFECTION | en |
dc.subject | BLOOD | en |
dc.subject | DOGS | en |
dc.subject | AMPLIFICATION | en |
dc.subject | DISEASE | en |
dc.subject | SAMPLES | en |
dc.subject | TESTS | en |
dc.subject | DNA | en |
dc.subject | Microbiology | en |
dc.subject | Veterinary Sciences | en |
dc.title | Evaluation of a serum-based PCR assay for the diagnosis of canine monocytic ehrlichiosis | en |
dc.type | journalArticle | en |
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