dc.creator | Margaritopoulos, J. T. | en |
dc.creator | Kouretas, D. | en |
dc.creator | Tsitsipis, J. A. | en |
dc.date.accessioned | 2015-11-23T10:38:53Z | |
dc.date.available | 2015-11-23T10:38:53Z | |
dc.date.issued | 2001 | |
dc.identifier.issn | 0258-851X | |
dc.identifier.uri | http://hdl.handle.net/11615/30703 | |
dc.description.abstract | We present here a modification of the original differential display approach using a single oligo(dT) primer for the reverse transcription reaction (instead of the various oligo(dT)NM primers that subdivide the pool of mRNAs) and a combination of 25-mer or 26-mer arbitrary primers together with 30-mer anchored primers for the PCR reaction. The PCR products are, then, efficiently separated in a non-denaturing polyacrylamide gel and the bands are visualized after staining with silver nitrate. The model for the development of our differential display approach was seven clones of an insect species: the aphid Myzus pesicae (Sulzer) (Homoptera: Aphididae). We believe that our modified differential display technique, with the efficient resolution of the DNA bands in a non-denaturing gel and staining with silver can be applied as an alternative non-radioactive detection of differentially expressed messages in various cell populations. In addition, the method could be used as a supplementary tool to other techniques for examining inter- and intraspecific genetic variation in aphids. | en |
dc.source | In Vivo | en |
dc.source.uri | <Go to ISI>://WOS:000170282800008 | |
dc.subject | mRNA | en |
dc.subject | differential display | en |
dc.subject | PCR | en |
dc.subject | aphids | en |
dc.subject | POLYMERASE CHAIN-REACTION | en |
dc.subject | MYZUS-PERSICAE | en |
dc.subject | NICOTIANAE HOMOPTERA | en |
dc.subject | APHIDIDAE | en |
dc.subject | DISCRIMINATION | en |
dc.subject | HEMIPTERA | en |
dc.subject | DISPLAY | en |
dc.subject | Medicine, Research & Experimental | en |
dc.title | A modified method for the detection of differentially expressed mRNAs without using radioactivity | en |
dc.type | journalArticle | en |