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A highly carbapenem-resistant Pseudomonas aeruginosa isolate with a novel bla(VIM-4)/bla(P1b) integron overexpresses two efflux pumps and lacks OprD
dc.creator | Maniati, M. | en |
dc.creator | Ikonomidis, A. | en |
dc.creator | Mantzana, P. | en |
dc.creator | Daponte, A. | en |
dc.creator | Maniatis, A. N. | en |
dc.creator | Pournaras, S. | en |
dc.date.accessioned | 2015-11-23T10:38:40Z | |
dc.date.available | 2015-11-23T10:38:40Z | |
dc.date.issued | 2007 | |
dc.identifier | 10.1093/jac/dkm126 | |
dc.identifier.issn | 0305-7453 | |
dc.identifier.uri | http://hdl.handle.net/11615/30617 | |
dc.description.abstract | Objectives: A Pseudomonas aeruginosa clinical isolate that exhibited high-level carbapenem resistance and produced metallo-beta-lactamase (MBL) was recovered from a Greek patient. This study was conducted to determine the underlying mechanisms that conferred the carbapenem resistance phenotype. Methods: MICs were determined by Etest and Etest MBL. PCR assays were performed for identification of bla(VIM-type), other antibiotic resistance and efflux pump genes and mapping of class 1 integrons. Expression of efflux pump genes was quantified by real-time PCR. Nucleotide sequencing was used to determine the bla(VIM) allele. The location of the MBL allele was investigated by mating experiments, plasmid analysis and hybridization studies. Results: The isolate was highly carbapenem-resistant (MICs of imipenem and meropenern were 512 and 128 mg/L, respectively) and multidrug-resistant. It harboured the P-lactamase genes bla(VIM-4) and bla(P1b) in a novel class 1 integron named InV4P1, and a second integron with aac(6')-lb and bla(OXA-35) gene cassettes. The isolate was deficient in porin OprD and overexpressed eff lux pumps MexAB-OprM and MexXY-OprM. Conjugation experiments failed to detect transferable MBL determinants, plasmids were not visualized and bla(VIM) was detected by PCR in the chromosomal band. Conclusions: Multiple carbapenem resistance mechanisms are demonstrated to coexist in a single A aeruginosa isolate and might confer the high-level carbapenem resistance. | en |
dc.source | Journal of Antimicrobial Chemotherapy | en |
dc.source.uri | <Go to ISI>://WOS:000248180200018 | |
dc.subject | PSE-1 | en |
dc.subject | In4P1 | en |
dc.subject | P. aeruginosa | en |
dc.subject | METALLO-BETA-LACTAMASE | en |
dc.subject | GENE | en |
dc.subject | OUTBREAK | en |
dc.subject | Infectious Diseases | en |
dc.subject | Microbiology | en |
dc.subject | Pharmacology & Pharmacy | en |
dc.title | A highly carbapenem-resistant Pseudomonas aeruginosa isolate with a novel bla(VIM-4)/bla(P1b) integron overexpresses two efflux pumps and lacks OprD | en |
dc.type | journalArticle | en |
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