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Digoxin and Adenosine Triphosphate Enhance the Functional Properties of Tissue-Engineered Cartilage
dc.creator | Makris, E. A. | en |
dc.creator | Huang, B. J. | en |
dc.creator | Hu, J. C. | en |
dc.creator | Chen-Izu, Y. | en |
dc.creator | Athanasiou, K. A. | en |
dc.date.accessioned | 2015-11-23T10:38:27Z | |
dc.date.available | 2015-11-23T10:38:27Z | |
dc.date.issued | 2015 | |
dc.identifier | 10.1089/ten.tea.2014.0360 | |
dc.identifier.issn | 1937-3341 | |
dc.identifier.uri | http://hdl.handle.net/11615/30526 | |
dc.description.abstract | Toward developing engineered cartilage for the treatment of cartilage defects, achieving relevant functional properties before implantation remains a significant challenge. Various chemical and mechanical stimuli have been used to enhance the functional properties of engineered musculoskeletal tissues. Recently, Ca2+-modulating agents have been used to enhance matrix synthesis and biomechanical properties of engineered cartilage. The objective of this study was to determine whether other known Ca2+ modulators, digoxin and adenosine triphosphate (ATP), can be employed as novel stimuli to increase collagen synthesis and functional properties of engineered cartilage. Neocartilage constructs were formed by scaffold-free self-assembling of primary bovine articular chondrocytes. Digoxin, ATP, or both agents were added to the culture medium for 1 h/day on days 10-14. After 4 weeks of culture, neocartilage properties were assessed for gross morphology, biochemical composition, and biomechanical properties. Digoxin and ATP were found to increase neocartilage collagen content by 52-110% over untreated controls, while maintaining proteoglycan content near native tissue values. Furthermore, digoxin and ATP increased the tensile modulus by 280% and 180%, respectively, while the application of both agents increased the modulus by 380%. The trends in tensile properties were found to correlate with the amount of collagen cross-linking. Live Ca2+ imaging experiments revealed that both digoxin and ATP were able to increase Ca2+ oscillations in monolayer-cultured chondrocytes. This study provides a novel approach toward directing neocartilage maturation and enhancing its functional properties using novel Ca2+ modulators. | en |
dc.source.uri | <Go to ISI>://WOS:000350549500003 | |
dc.subject | COLLAGEN CROSS-LINKING | en |
dc.subject | BOVINE ARTICULAR-CARTILAGE | en |
dc.subject | DYNAMIC COMPRESSION | en |
dc.subject | INTRACELLULAR CALCIUM | en |
dc.subject | CHONDROITINASE-ABC | en |
dc.subject | GENE-EXPRESSION | en |
dc.subject | CHONDROCYTE | en |
dc.subject | MECHANOTRANSDUCTION | en |
dc.subject | MECHANICAL-PROPERTIES | en |
dc.subject | TENSILE PROPERTIES | en |
dc.subject | ATP | en |
dc.subject | Cell & Tissue Engineering | en |
dc.subject | Biotechnology & Applied Microbiology | en |
dc.subject | Cell | en |
dc.subject | Biology | en |
dc.title | Digoxin and Adenosine Triphosphate Enhance the Functional Properties of Tissue-Engineered Cartilage | en |
dc.type | journalArticle | en |
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