Mostra i principali dati dell'item

dc.creatorLiaskos, C.en
dc.creatorSpyrou, V.en
dc.creatorRoggenbuck, D.en
dc.creatorAthanasiou, L. V.en
dc.creatorOrfanidou, T.en
dc.creatorMavropoulos, A.en
dc.creatorReinhold, D.en
dc.creatorRigopoulou, E. I.en
dc.creatorAmiridis, G. S.en
dc.creatorBillinis, C.en
dc.creatorBogdanos, D. P.en
dc.date.accessioned2015-11-23T10:37:59Z
dc.date.available2015-11-23T10:37:59Z
dc.date.issued2013
dc.identifier10.3109/08916934.2013.786047
dc.identifier.issn0891-6934
dc.identifier.urihttp://hdl.handle.net/11615/30338
dc.description.abstractMycobacterium avium subspecies paratuberculosis (MAP) induces paratuberculosis (ptb) in ruminants and has clinical and histological features resebling Crohn's disease (CD). Pancreatic autoantibodies (PAB) targeting glycoprotein 2 (GP2) are specifically found in CD, but it is currently unknown whether these autoantibodies can be found in ruminants with ptb. IgG anti-MAP and anti-GP2 antibodies were tested by ELISA in 286 ruminants (212 sheep and 74 cattle). PAB testing was performed by indirect immunofluorescence (IF) using anti-sheep or anti-cattle specific antisera. PCR analysis confirmed the presence of MAP in anti-MAP positive samples. Anti-GP2 antibodies were more prevalent in anti-MAP antibody positive (26.9%) than in anti-MAP negative ruminants (8.7%, p<0.001). Anti-GP2 antibodies were found in 16/70 (22.9%) anti-MAP positive sheep compared to 10/142 (7%, p=0.001) anti-MAP antibody negative and in anti-MAP positive cattle than in negative counterparts (5/8 versus 8/66, p=0.003). Absorbance values for anti-GP2 antibodies were higher in cattle than in sheep (mean 21 AU/mL +/- 25.45D versus 12.2 AU/mL +/- 23 SD, p<0.001). There was no correlation between anti-GP2 and anti-MAP antibody concentrations. Anti-GP2 antibodies persisted up to 1/1000 and showed the characteristic IIF pancreatic pattern seen by anti-GP2 antibody positive CD samples. This is the first study to demonstrate the presence of CD-specific GP2-reactive pancreatic autoantibodies in MAP-infected ruminants. Our data suggest that CD and ptb are characterised by an antigen-driven loss of immunological tolerance to GP2, implying commonalities in the immunopathogenesis of the human and ruminant inflammatory bowel disorder.en
dc.source.uri<Go to ISI>://WOS:000324079700006
dc.subjectAutoantibodyen
dc.subjectautoimmunityen
dc.subjectinfectionen
dc.subjectinflammatory bowel diseaseen
dc.subjectintestineen
dc.subjectAVIUM SUBSPECIES PARATUBERCULOSISen
dc.subjectINFLAMMATORY-BOWEL-DISEASEen
dc.subjectEXOCRINEen
dc.subjectPANCREASen
dc.subjectGLYCOPROTEIN 2en
dc.subjectJOHNES-DISEASEen
dc.subjectMYCOBACTERIUMen
dc.subjectANTIBODIESen
dc.subjectMETAANALYSISen
dc.subjectREACTIVITYen
dc.subjectDIAGNOSISen
dc.subjectImmunologyen
dc.titleCrohn's disease-specific pancreatic autoantibodies are specifically present in ruminants with paratuberculosis: Implications for the pathogenesis of the human diseaseen
dc.typejournalArticleen


Files in questo item

FilesDimensioneFormatoMostra

Nessun files in questo item.

Questo item appare nelle seguenti collezioni

Mostra i principali dati dell'item