dc.creator | Kouvaras, E. | en |
dc.creator | Papandreou, C. N. | en |
dc.creator | Daliani, D. D. | en |
dc.creator | Athanasiadis, A. | en |
dc.creator | Koukoulis, G. K. | en |
dc.date.accessioned | 2015-11-23T10:36:58Z | |
dc.date.available | 2015-11-23T10:36:58Z | |
dc.date.issued | 2012 | |
dc.identifier | 10.1016/j.breast.2011.07.009 | |
dc.identifier.issn | 0960-9776 | |
dc.identifier.uri | http://hdl.handle.net/11615/30003 | |
dc.description.abstract | One presumed drawback of performing fluorescence in situ hybridization on routine tissue sections for HER-2 status evaluation in breast carcinomas is nuclear truncation. Therefore, HER-2/CEP 17 ratios were compared in routine (4 mu m) vs. thicker (15 gm) tissue sections. Additionally, the distances of both signals from the nuclear center were measured by three-dimensional image analysis. HER-2 and CEP 17 signals' number increased in thick sections; however, HER-2/CEP 17 ratios were decreased. This could be attributed to a preferential increase in CEP17 signals explained by their more peripheral localization and apparent "loss" in truncated nuclei. The aforementioned decrease of HER-2 ratios did not alter HER-2 status except in cases in the equivocal category where it changed from equivocal to non-amplified. Thus, at least a subset of the equivocal cases could represent an artifactual increase of HER-2 ratio related to nuclear truncation and loss of peripheral CEP 17 signals in routine sections. (C) 2011 Elsevier Ltd. All rights reserved. | en |
dc.source.uri | <Go to ISI>://WOS:000300925600005 | |
dc.subject | Breast | en |
dc.subject | FISH | en |
dc.subject | HER-2 | en |
dc.subject | Equivocal | en |
dc.subject | IN-SITU HYBRIDIZATION | en |
dc.subject | METASTATIC BREAST-CANCER | en |
dc.subject | MONOCLONAL-ANTIBODY | en |
dc.subject | GUIDELINES | en |
dc.subject | ONCOGENE | en |
dc.subject | CHEMOTHERAPY | en |
dc.subject | ABERRATIONS | en |
dc.subject | EFFICACY | en |
dc.subject | SAFETY | en |
dc.subject | Oncology | en |
dc.subject | Obstetrics & Gynecology | en |
dc.title | Comparative study of spatial localization of HER-2 and CEP17 signals and of HER-2/CEP17 ratios, in "thin" and "thick" tissue sections | en |
dc.type | journalArticle | en |