dc.creator | Kouritas, V. K. | en |
dc.creator | Ioannou, M. | en |
dc.creator | Desimonas, N. | en |
dc.creator | Gourgoulianis, K. I. | en |
dc.creator | Molyvdas, P. A. | en |
dc.creator | Hatzoglou, C. | en |
dc.date.accessioned | 2015-11-23T10:36:22Z | |
dc.date.available | 2015-11-23T10:36:22Z | |
dc.date.issued | 2013 | |
dc.identifier | 10.1016/j.ghir.2012.10.002 | |
dc.identifier.issn | 1096-6374 | |
dc.identifier.uri | http://hdl.handle.net/11615/29860 | |
dc.description.abstract | Objective: The effect of IGF-1 in the human pleural permeability and the underlying mechanisms involved were investigated. Design: Specimens from thoracic surgical patients were mounted in Ussing chambers. Solutions containing IGF-1 (1 nM-100 nM) and IGF-1 Receptor Inhibitor (1 mu M), amiloride 10 mu M (Na+ channel blocker) and ouabain 1 mM (Na+-K+ pump inhibitor) were used in order to investigate receptor and ion transporter involvement respectively. Trans-mesothelial Resistance (R-TM) across the pleural membrane was determined as a permeability indicator. Immunohistochemistry for IGF-1 receptors was performed. Results: IGF-1 increased R-TM when added on the interstitial surface for all concentrations (p = .008, 1 nM-100 nM) and decreased it on the mesothelial surface for higher concentrations (p = .046, 100 nM). Amiloride and ouabain inhibited this effect. The IGF-1 Receptor Inhibitor also totally inhibited this effect. Immonuhistochemistry demonstrated the presence of IGF-1 receptors in the pleura. Conclusions: It is concluded that IGF-1 changes the electrophysiology of the human parietal pleura by hindering the normal ion transportation and therefore the pleural fluid recycling process. This event is achieved after IGF-1 interaction with its receptor which is present in the human pleura. (C) 2012 Elsevier Ltd. All rights reserved. | en |
dc.source | Growth Hormone & Igf Research | en |
dc.source.uri | <Go to ISI>://WOS:000316711400002 | |
dc.subject | IGF | en |
dc.subject | 1, IGF | en |
dc.subject | 1 Receptor | en |
dc.subject | Pleura | en |
dc.subject | Human | en |
dc.subject | Permeability | en |
dc.subject | GROWTH-FACTOR-I | en |
dc.subject | NA+-TRANSPORT | en |
dc.subject | CAPILLARY-PERMEABILITY | en |
dc.subject | BINDING | en |
dc.subject | PROTEIN-2 | en |
dc.subject | PROSTATE-CANCER | en |
dc.subject | PLASMA-LEVELS | en |
dc.subject | LUNG-CANCER | en |
dc.subject | RAT-KIDNEY | en |
dc.subject | INSULIN | en |
dc.subject | CELLS | en |
dc.subject | Cell Biology | en |
dc.subject | Endocrinology & Metabolism | en |
dc.title | IGF-1 alters the human parietal pleural electrochemical profile by inhibiting ion trans-cellular transportation after interaction with its receptor | en |
dc.type | journalArticle | en |