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Association of soybean-based extenders with field fertility of stored ram (Ovis aries) semen: A randomized double-blind parallel group design
dc.creator | Khalifa, T. | en |
dc.creator | Lymberopoulos, A. | en |
dc.creator | Theodosiadou, E. | en |
dc.date.accessioned | 2015-11-23T10:34:47Z | |
dc.date.available | 2015-11-23T10:34:47Z | |
dc.date.issued | 2013 | |
dc.identifier | 10.1016/j.theriogenology.2012.11.009 | |
dc.identifier.issn | 0093-691X | |
dc.identifier.uri | http://hdl.handle.net/11615/29407 | |
dc.description.abstract | Two consecutive randomized double-blind field fertility experiments were conducted over a 4-month period and aimed at evaluating the association of two commercial soybean lecithin-based extenders (AndroMed [Minitub, Tiefenbach, Germany] and BioXcell [IMV Technologies, L'Aigle, France]) with pregnancy rates of chilled-stored (CS) and frozen-thawed (FT) ram semen. Semen samples with more than 2 x 10(9) sperm per mL and 70% progressive motile spermatozoa were collected via an artificial vagina from twelve proven fertile Chios rams, split-diluted with the above mentioned extenders, packaged in 0.25 mL straws and either stored at 5 +/- 1 degrees C for 30 to 36 hours or frozen and thawed. Non-lactating multiparous ewes were inseminated in progestagen-synchronized estrus either with CS (AndroMed: N = 212 and BioXcell: N = 206; intracervical AI) or with FT (AndroMed: N = 114 and BioXcell: N = 92; laparoscopic intrauterine AI) semen. Ovulation was confirmed in all ewes based on determination of blood plasma progesterone (>1 ng/mL) 8 days post AI. Ewes were screened for pregnancy diagnosis by transabdominal ultrasonography 65 days post AI. BioXcell was superior to AndroMed in preserving the fertilizing potential of CS (P < 0.05) and FT (P < 0.005) semen. In AndroMed-stored semen, young rams (1.5-2.5 years old, N = 8) had a pregnancy rate (59.1%; 124/210) lower than that (72.4%; 84/116) of mature rams (4.5 to 5.5 years, N = 4; P < 0.025). Compared with AndroMed extender, processing of young ram semen in BioXcell extender improved pregnancy rates of CS (66.7%; 88/132 vs. 83.9%; 94/112; P < 0.005) and FT (46.2%; 36/78 vs. 71.0%; 44/62; P < 0.01) spermatozoa. Both extenders were similarly effective in preserving pregnancy rates of mature ram semen (P > 0.05). Ram-by-extender interactions were significant for pregnancy rates of CS and FT semen. Irrespective of extenders, overall pregnancy rates after intracervical and intrauterine AI were 75.1% and 62.2%, respectively (P < 0.001). In conclusion, BioXcell is a suitable extender for short- and long-term storage of ram semen. Selection of the ewes, farms, and extenders for intracervical AI programs can contribute to satisfactory fertility rates with semen preserved more than 24 hours at 5 degrees C. (c) 2013 Elsevier Inc. All rights reserved. | en |
dc.source | Theriogenology | en |
dc.source.uri | <Go to ISI>://WOS:000313933800015 | |
dc.subject | Sheep | en |
dc.subject | Spermatozoa | en |
dc.subject | AndroMed | en |
dc.subject | BioXcell | en |
dc.subject | Artificial insemination | en |
dc.subject | Pregnancy rate | en |
dc.subject | FROZEN-THAWED SEMEN | en |
dc.subject | LAPAROSCOPIC ARTIFICIAL-INSEMINATION | en |
dc.subject | IN-VITRO | en |
dc.subject | FERTILIZATION | en |
dc.subject | BOVINE SERUM-ALBUMIN | en |
dc.subject | CERVICAL INSEMINATION | en |
dc.subject | INTRAUTERINE | en |
dc.subject | INSEMINATION | en |
dc.subject | SEASONAL-VARIATION | en |
dc.subject | SEMINAL PLASMA | en |
dc.subject | EGG-YOLK | en |
dc.subject | OVULATORY | en |
dc.subject | ACTIVITY | en |
dc.subject | Reproductive Biology | en |
dc.subject | Veterinary Sciences | en |
dc.title | Association of soybean-based extenders with field fertility of stored ram (Ovis aries) semen: A randomized double-blind parallel group design | en |
dc.type | journalArticle | en |
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