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Modulating effect of leptin on basal and follicle stimulating hormone stimulated steroidogenesis in cultured human lutein granulosa cells
dc.creator | Karamouti, M. | en |
dc.creator | Kollia, P. | en |
dc.creator | Kallitsaris, A. | en |
dc.creator | Vamvakopoulos, N. | en |
dc.creator | Kollios, G. | en |
dc.creator | Messinis, I. E. | en |
dc.date.accessioned | 2015-11-23T10:33:14Z | |
dc.date.available | 2015-11-23T10:33:14Z | |
dc.date.issued | 2009 | |
dc.identifier.issn | 0391-4097 | |
dc.identifier.uri | http://hdl.handle.net/11615/29020 | |
dc.description.abstract | Background: In vitro data have shown conflicting results in terms of the effect of leptin on granulosa cells steroidogenesis. Aim: The aim of the present study was to investigate the effect of low and high doses of leptin on basal and FSH-induced steroids secretion by human luteinized granulosa cells in culture. Materials and methods: Granulosa cells were obtained from normal women undergoing in vitro fertilization (IVF) treatment and were cultured in serum-free conditions for 72 h. A one-way analysis of variance design was set to study the effect of leptin on basal and FSH-induced steroidogenesis. Results: Leptin affected basal estradiol and progesterone secretion in a dose-related manner. In particular, leptin at low concentrations stimulated the secretion of estradiol (11 and 10 ng/ml) and progesterone (10 ng/ml), while at a high concentration (100 ng/ml) it suppressed the secretion of both steroids. A dose-related effect of leptin on FSH-induced steroidogenesis was not evident, since only the suppressive effect of the high concentration of leptin (100 ng/ml) reached statistical significance for both steroids. Conclusions: These results demonstrate that leptin affects the secretion of steroids in luteinized granulosa cells in a dose-dependent manner. Although a physiological role for leptin is possible, it is suggested that this protein is a mediator of negative rather than positive influential interactions on ovarian function that may compromise fertility. (J. Endocrinol. Invest. 32: 415-419, 2009) (C) 2009, Editrice Kurtis | en |
dc.source.uri | <Go to ISI>://WOS:000270471500006 | |
dc.subject | Culture | en |
dc.subject | human | en |
dc.subject | leptin | en |
dc.subject | lutein granulosa cells | en |
dc.subject | ovarian steroidogenesis | en |
dc.subject | GROWTH-FACTOR-I | en |
dc.subject | OBESE GENE-PRODUCT | en |
dc.subject | AROMATASE-ACTIVITY | en |
dc.subject | NORMAL WOMEN | en |
dc.subject | HUMAN OVARY | en |
dc.subject | INSULIN | en |
dc.subject | PROGESTERONE | en |
dc.subject | EXPRESSION | en |
dc.subject | FERTILIZATION | en |
dc.subject | ESTRADIOL | en |
dc.subject | Endocrinology & Metabolism | en |
dc.title | Modulating effect of leptin on basal and follicle stimulating hormone stimulated steroidogenesis in cultured human lutein granulosa cells | en |
dc.type | journalArticle | en |
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