Growth hormone, insulin-like growth factor I, and leptin interaction in human cultured lutein granulosa cells steroidogenesis
dc.creator | Karamouti, M. | en |
dc.creator | Kollia, P. | en |
dc.creator | Kallitsaris, A. | en |
dc.creator | Vamvakopoulos, N. | en |
dc.creator | Kollios, G. | en |
dc.creator | Messinis, I. E. | en |
dc.date.accessioned | 2015-11-23T10:33:14Z | |
dc.date.available | 2015-11-23T10:33:14Z | |
dc.date.issued | 2008 | |
dc.identifier | 10.1016/j.fertnstert.2007.08.076 | |
dc.identifier.issn | 150282 | |
dc.identifier.uri | http://hdl.handle.net/11615/29019 | |
dc.description.abstract | Objective: To investigate the effect of leptin on the actions of growth hormone (GH) and insulin-like growth factor-I (IGF-I) in ovarian steroidogenesis in vitro. Design: In vitro cell culture study. Setting: Research laboratory of a university hospital. Patient(s): Luteinized granulosa cells (GC) were obtained from 20 healthy women undergoing ovarian stimulation for IVF treatment. Intervention(s): Granulosa cells were cultured in serum free conditions for 72 hours with either GH or IGF-I, or their combination, in the absence or presence of leptin (1, 10, and 100 ng/mL). Main Outcome Measure(s): At 24 and 72 hours of the culture estradiol (E2) and progesterone (P) concentrations were measured in culture supernatants by enzyme immunoassays. Result(s): Leptin at the doses of 1 and 10 ng/mL significantly stimulated, whereas at the dose of 100 ng/mL it significantly suppressed E2 and P production as compared to control. Estradiol production was significantly stimulated by both GH (72 hours) and IGF-I (24 and 72 hours) and that of P only by IGF-I (72 hours). Leptin at the low doses enhanced, whereas at the high dose it suppressed the effect of GH or IGF-I on E2 and P synthesis. Conclusion(s): These results demonstrate for the first time that leptin can modulate the effect of GH on steroids production by human luteinized GC in culture. © 2008 American Society for Reproductive Medicine. | en |
dc.source.uri | http://www.scopus.com/inward/record.url?eid=2-s2.0-52949126050&partnerID=40&md5=fccc643d13f1d3bb3e15932be8d446cc | |
dc.subject | culture | en |
dc.subject | GH | en |
dc.subject | granulosa cells | en |
dc.subject | human | en |
dc.subject | IGF-I | en |
dc.subject | Leptin | en |
dc.subject | ovarian steroidogenesis | en |
dc.subject | estrogen | en |
dc.subject | growth hormone | en |
dc.subject | progesterone | en |
dc.subject | somatomedin C | en |
dc.subject | adult | en |
dc.subject | article | en |
dc.subject | cell culture | en |
dc.subject | clinical article | en |
dc.subject | enzyme immunoassay | en |
dc.subject | female | en |
dc.subject | granulosa cell | en |
dc.subject | hormone synthesis | en |
dc.subject | human cell | en |
dc.subject | ovulation induction | en |
dc.subject | priority journal | en |
dc.subject | protein function | en |
dc.subject | protein protein interaction | en |
dc.subject | steroidogenesis | en |
dc.subject | Cells, Cultured | en |
dc.subject | Estradiol | en |
dc.subject | Human Growth Hormone | en |
dc.subject | Humans | en |
dc.subject | Insulin-Like Growth Factor I | en |
dc.subject | Lutein | en |
dc.subject | Ovary | en |
dc.subject | Steroids | en |
dc.title | Growth hormone, insulin-like growth factor I, and leptin interaction in human cultured lutein granulosa cells steroidogenesis | en |
dc.type | journalArticle | en |
Files in this item
Files | Size | Format | View |
---|---|---|---|
There are no files associated with this item. |