dc.creator | Karadonta, A. V. | en |
dc.creator | Alexandrakis, M. G. | en |
dc.creator | Kourelis, T. V. | en |
dc.creator | Manola, A. | en |
dc.creator | Garlemou, K. | en |
dc.creator | Stathakis, N. E. | en |
dc.creator | Kyriakou, D. | en |
dc.date.accessioned | 2015-11-23T10:32:54Z | |
dc.date.available | 2015-11-23T10:32:54Z | |
dc.date.issued | 2009 | |
dc.identifier.issn | 0394-6320 | |
dc.identifier.uri | http://hdl.handle.net/11615/28941 | |
dc.description.abstract | Myelodysplastic Syndrome (MDS) cells present genetic instability and dysregulation of the gene C3ORF9, which was isolated from an MDS cDNA library and codes for a putative protein. We studied the expression of C3ORF9 in MDS syndromes to contribute to the understanding of the pathophysiology of MDS. One hundred and thirty-one patients and 35 healthy controls were involved in our study. Bone marrow aspirates and isolated CD34+ cells were used. Gene expression was estimated by quantitative PCR. C3ORF9 was found to be down-regulated in patients with CMML compared to the controls (p<0.01). There was no difference between RARS and the controls (p=0.1), while increased expression was found in RA, RAEB and RAEB-T (p<0.01 for all). No mutations or polymorphism were detected in our population. CD34+ cells expressed higher levels of C3ORF9 (p<0.01) in patients. The gene expression was correlated to the percentage of + cells in RAEB and RAEB-T (r=0.64). The altered C3ORF9 expression was possibly due to different gene regulation in these patients and/or to the increased CD34+ cells. | en |
dc.source.uri | <Go to ISI>://WOS:000271125100025 | |
dc.subject | C3ORF9 | en |
dc.subject | mds | en |
dc.subject | chromosome abnormalities | en |
dc.subject | PHENOL-CHLOROFORM EXTRACTION | en |
dc.subject | SINGLE-STEP METHOD | en |
dc.subject | RNA ISOLATION | en |
dc.subject | DNA | en |
dc.subject | ACID | en |
dc.subject | PROTEINS | en |
dc.subject | REAGENT | en |
dc.subject | CLONING | en |
dc.subject | YEAST | en |
dc.subject | Immunology | en |
dc.subject | Pathology | en |
dc.subject | Pharmacology & Pharmacy | en |
dc.title | ABNORMAL EXPRESSION OF C3ORF9 GENE IN PATIENTS WITH MYELODYSPLASTIC SYNDROMES | en |
dc.type | journalArticle | en |