dc.creator | Ikonomidis, A. | en |
dc.creator | Neou, E. | en |
dc.creator | Gogou, V. | en |
dc.creator | Vrioni, G. | en |
dc.creator | Tsakris, A. | en |
dc.creator | Pournaras, S. | en |
dc.date.accessioned | 2015-11-23T10:30:19Z | |
dc.date.available | 2015-11-23T10:30:19Z | |
dc.date.issued | 2009 | |
dc.identifier | 10.1128/jcm.00959-09 | |
dc.identifier.issn | 0095-1137 | |
dc.identifier.uri | http://hdl.handle.net/11615/28559 | |
dc.description.abstract | The characteristics of carbapenem heteroresistance were studied in 14 apparently carbapenem-susceptible Acinetobacter baumannii isolates. The MICs for carbapenems were determined, and the isolates were genotyped by pulsed-field gel electrophoresis (PFGE) and sequence typing (ST). Population analysis, testing of the stability of the heteroresistant subpopulations, and time-killing assays were performed. The agar dilution MICs of both imipenem and meropenem for the native isolates ranged from 0.25 to 4 mg/liter. The isolates belonged to nine PFGE types and exhibited seven ST allelic profiles. Population analysis revealed subpopulations that grew in the presence of imipenem at concentrations of up to 8 mg/liter and meropenem at concentrations of up to 32 mg/liter. The meropenem-heteroresistant subpopulations of 11 isolates exhibited stable resistance with MICs that ranged from 16 to >32 mg/liter; their PFGE profiles were identical to those of the native isolates. Time-kill assays with meropenem revealed less pronounced killing for 10 isolates. These findings indicate that meropenem pressure can produce meropenem-heteroresistant subpopulations that might subsequently select for highly resistant strains. | en |
dc.source | Journal of Clinical Microbiology | en |
dc.source.uri | <Go to ISI>://WOS:000272230500037 | |
dc.subject | METALLO-BETA-LACTAMASE | en |
dc.subject | PSEUDOMONAS-AERUGINOSA | en |
dc.subject | MOLECULAR EPIDEMIOLOGY | en |
dc.subject | RESISTANT | en |
dc.subject | GENE | en |
dc.subject | OUTBREAK | en |
dc.subject | IMIPENEM | en |
dc.subject | CLONES | en |
dc.subject | OXA-58 | en |
dc.subject | HOSPITALS | en |
dc.subject | Microbiology | en |
dc.title | Heteroresistance to Meropenem in Carbapenem-Susceptible Acinetobacter baumannii | en |
dc.type | journalArticle | en |