Double reactivity against actin and alpha-actinin defines a severe form of autoimmune hepatitis type 1
dc.creator | Gueguen, P. | en |
dc.creator | Dalekos, G. | en |
dc.creator | Nousbaum, J. B. | en |
dc.creator | Zachou, K. | en |
dc.creator | Putterman, C. | en |
dc.creator | Youinou, P. | en |
dc.creator | Renaudineau, Y. | en |
dc.date.accessioned | 2015-11-23T10:29:17Z | |
dc.date.available | 2015-11-23T10:29:17Z | |
dc.date.issued | 2006 | |
dc.identifier | 10.1007/s10875-006-9045-z | |
dc.identifier.issn | 0271-9142 | |
dc.identifier.uri | http://hdl.handle.net/11615/28243 | |
dc.description.abstract | Anti-filamentous actin antibodies characterize autoimmune hepatitis type 1 (AIH-1). Recently, the binding domain of alpha-actinin on actin was shown to be a predominant epitope. To test this reactivity, an anti-alpha-actinin enzyme-linked immunosorbent assay was developed, and positivity confirmed by Western blot. Anti-alpha-actinin antibody was found in 21/50 (42%) of AIH-1 patients, compared with 52/401 (12.9%) of liver disease control patients, and with 6/200 (6%) of blood donors. Anti-filamentous and anti-alpha-actinin activities were found specifically together in 66% of anti-filamentous-positive AIH-1 patients. This combination of specificities reflected clinical and histological disease activity, short duration and absence of treatment. Finally, using an actin-alpha-actinin complex assay, the binding of anti-filamentous actin to alpha-actinin-binding domain on actin was demonstrated, as well as that of anti-alpha-actinin on the actin-binding domain of alpha-actinin. Thus, the frequent combination of anti-filamentous and anti-alpha-actinin antibodies seems to be the hallmark of activity in AIH-1. | en |
dc.source.uri | <Go to ISI>://WOS:000242130900001 | |
dc.subject | autoimmunity | en |
dc.subject | anti-smooth-muscle antibodies | en |
dc.subject | active disease | en |
dc.subject | binding | en |
dc.subject | domain | en |
dc.subject | anti-ssDNA | en |
dc.subject | ANTI-DNA ANTIBODIES | en |
dc.subject | DOUBLE-STRANDED DNA | en |
dc.subject | ANTIACTIN ANTIBODIES | en |
dc.subject | LUPUS | en |
dc.subject | NEPHRITIS | en |
dc.subject | AUTOANTIBODIES | en |
dc.subject | TARGET | en |
dc.subject | SERA | en |
dc.subject | IMMUNOFLUORESCENCE | en |
dc.subject | ALPHA-ACTININ-2 | en |
dc.subject | EXPRESSION | en |
dc.subject | Immunology | en |
dc.title | Double reactivity against actin and alpha-actinin defines a severe form of autoimmune hepatitis type 1 | en |
dc.type | journalArticle | en |
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