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Macrolide resistance determinants among Streptococcus pneumoniae isolates from carriers in Central Greece
dc.creator | Grivea, I. N. | en |
dc.creator | Sourla, A. | en |
dc.creator | Ntokou, E. | en |
dc.creator | Chryssanthopoulou, D. C. | en |
dc.creator | Tsantouli, A. G. | en |
dc.creator | Syrogiannopoulos, G. A. | en |
dc.date.accessioned | 2015-11-23T10:29:16Z | |
dc.date.available | 2015-11-23T10:29:16Z | |
dc.date.issued | 2012 | |
dc.identifier | 10.1186/1471-2334-12-255 | |
dc.identifier.issn | 1471-2334 | |
dc.identifier.uri | http://hdl.handle.net/11615/28236 | |
dc.description.abstract | Background: We sought to characterize the temporal trends in nasopharyngeal carriage of macrolide-resistant pneumococci during a period with increased heptavalent pneumococcal conjugate vaccine (PCV7) coverage in Central Greece. Methods: Streptococcus pneumoniae isolates were recovered from 2649 nasopharyngeal samples obtained from day-care center attendees in Central Greece during 2005-2009. A phenotypic and genotypic analysis of the isolates was performed, including the identification of macrolide resistance genes mef(A), subclasses mef(A) and mef(E), as well as erm(B). Results: Of the 1105 typeable S. pneumoniae isolates, 265 (24%) were macrolide-resistant; 22% in 2005, 33.3% in 2006, 23.7% in 2007, and 20.5% in 2009 (P=0.398). Among these macrolide-resistant pneumococci, 28.5% possessed erm(B), 24.3% erm(B)+mef(E), 41.8% mef(E), and 5.3% mef(A). A mef gene as the sole resistance determinant was carried by 31% of macrolide-resistant isolates belonging to PCV7 serotypes and 75.8% of the non-PCV7 serotypes. Across the 4 annual surveillances, pneumococci carrying mef(A) gradually disappeared, whereas serotype 19F isolates carrying both erm(B) and mef(E) persisted without significant yearly fluctuations. Among isolates belonging to non-PCV7 serotypes, macrolide-resistance was observed in those of serotypes 6A, 19A, 10A, 15A, 15B/C, 35F, 35A, and 24F. In 2009, ie 5 years after the introduction of PCV7 in our country, 59% of macrolide-resistant pneumococci belonged to non-PCV7 serotypes. Conclusions: Across the study period, the annual frequency of macrolide- resistant isolates did not change significantly, but in 2009 a marked shift to non-PCV7 serotypes occurred. Overall, more than half of the macrolide- resistant isolates possessed erm(B) either alone or in combination with mef(E). erm(B) dominated among isolates belonging to PCV7 serotypes, but not among those of non-PCV7 serotypes. | en |
dc.source | Bmc Infectious Diseases | en |
dc.source.uri | <Go to ISI>://WOS:000310353100001 | |
dc.subject | PNEUMOCOCCAL CONJUGATE VACCINE | en |
dc.subject | DAY-CARE-CENTERS | en |
dc.subject | EFFLUX GENE MEF(A) | en |
dc.subject | ACUTE OTITIS-MEDIA | en |
dc.subject | ERYTHROMYCIN RESISTANCE | en |
dc.subject | NASOPHARYNGEAL CARRIAGE | en |
dc.subject | MOLECULAR EPIDEMIOLOGY | en |
dc.subject | M PHENOTYPE | en |
dc.subject | CHILDREN | en |
dc.subject | SEROTYPES | en |
dc.subject | Infectious Diseases | en |
dc.title | Macrolide resistance determinants among Streptococcus pneumoniae isolates from carriers in Central Greece | en |
dc.type | journalArticle | en |
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