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Enzyme-linked immunosorbent assay for pharmacological studies targeting hypoxia-inducible factor 1 alpha
dc.creator | Formento, J. L. | en |
dc.creator | Berra, E. | en |
dc.creator | Ferrua, B. | en |
dc.creator | Magne, N. | en |
dc.creator | Simos, G. | en |
dc.creator | Brahimi-Horn, C. | en |
dc.creator | Pouyssegur, J. | en |
dc.creator | Milano, G. | en |
dc.date.accessioned | 2015-11-23T10:26:38Z | |
dc.date.available | 2015-11-23T10:26:38Z | |
dc.date.issued | 2005 | |
dc.identifier | 10.1128/cdli.12.5.660-664.2005 | |
dc.identifier.issn | 1071-412X | |
dc.identifier.uri | http://hdl.handle.net/11615/27509 | |
dc.description.abstract | Hypoxia-inducible factor 1 (HIF-1) activates the transcription of a wide range of genes related to oxygen delivery and metabolic adaptation under hypoxic (low-oxygen) conditions. HIF-1 is, in fact, a heterodimer of two subunits, HIF-1 alpha and HIF-1 beta. The only analytical methods available for measuring HIF-1 alpha levels in tumors are immunohistochemistry and Western blotting. Immunohistochemistry has the advantage of allowing the identification and direct examination of HIF-1 alpha-expressing cells, but has the intrinsic limitation, as for Western blotting, of being nonquantitative. We developed and validated an enzyme-linked immunosorbent assay (ELISA) approach to measure HIF-1 alpha levels in cultured tumor cell lines in vitro. HIF-1 alpha was expressed in thirteen tumor cell lines grown under hypoxic conditions; however, the levels differed strongly between cell lines. These data point to intrinsic differences between cell lines for the induction of HIF-1 alpha under hypoxic conditions. The ELISA developed in the present study is thus an interesting alternative to other analytical methods used to measure HIF-1 alpha protein levels and should be useful in preclinical pharmacological studies targeting HIF-1 alpha. | en |
dc.source.uri | <Go to ISI>://WOS:000231197000015 | |
dc.subject | FACTOR 1-ALPHA | en |
dc.subject | CANCER | en |
dc.subject | EXPRESSION | en |
dc.subject | HIF-1 | en |
dc.subject | HIF-1-ALPHA | en |
dc.subject | PROGNOSIS | en |
dc.subject | RADIOTHERAPY | en |
dc.subject | CARCINOMA | en |
dc.subject | THERAPY | en |
dc.subject | TISSUE | en |
dc.subject | Immunology | en |
dc.subject | Infectious Diseases | en |
dc.subject | Microbiology | en |
dc.title | Enzyme-linked immunosorbent assay for pharmacological studies targeting hypoxia-inducible factor 1 alpha | en |
dc.type | journalArticle | en |
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