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Strain-Specific Sensitivity Estimates of Mycobacterium avium subsp paratuberculosis Culture in Greek Sheep and Goats
dc.creator | Florou, M. | en |
dc.creator | Leontides, L. | en |
dc.creator | Kostoulas, P. | en |
dc.creator | Billinis, C. | en |
dc.creator | Sofia, M. | en |
dc.date.accessioned | 2015-11-23T10:26:35Z | |
dc.date.available | 2015-11-23T10:26:35Z | |
dc.date.issued | 2009 | |
dc.identifier | 10.1111/j.1863-2378.2008.01179.x | |
dc.identifier.issn | 1863-1959 | |
dc.identifier.uri | http://hdl.handle.net/11615/27479 | |
dc.description.abstract | The requirements for the isolation of Mycobacterium avium subsp. paratuberculosis (Map) may be related to the strain-type [sheep (S)- or cattle (C)-type] and not to the host. The objective of this paper was to estimate and compare strain- and biological sample (faeces or pooled-tissue) - specific sensitivities (Ses) of two solid culture media, Herrold's egg yolk medium (HEYM) and Lowenstein-Jensen (LJ) medium, for the isolation of Map from Greek dairy sheep and goats. From 400 faecal samples collected from sub-clinically infected sheep and goats of four flocks and from 214 pooled-tissue samples (142 from sheep and 72 from goats) collected, at the abattoir, from > 1-year-old routinely slaughtered animals, with gross pathology suggestive of paratuberculosis, we isolated 34 Map strains. Of those, by the IS1311 PCR, 18 were categorized into the C-type and nine into the S-type; seven were not typed. We used a Bayesian approach to estimate the strain-specific Ses. Se(HEYM-C-faecal) = 17% (95% credible interval: 7, 40) was higher than Se(HEYM-S-faecal) = 2% (0.3, 11). Also, Se(HEYM-C-faecal) was higher than Se(LJ-C-faecal) = 4% (1, 12). In pooled-tissue samples, the strain-specific Ses did not differ between the two media. | en |
dc.source | Zoonoses and Public Health | en |
dc.source.uri | <Go to ISI>://WOS:000262478400007 | |
dc.subject | Ovine and caprine paratuberculosis | en |
dc.subject | culture | en |
dc.subject | Bayesian estimation | en |
dc.subject | strain-specific sensitivity | en |
dc.subject | MEDIA | en |
dc.subject | OVINE | en |
dc.subject | ELISA | en |
dc.subject | Public, Environmental & Occupational Health | en |
dc.subject | Infectious Diseases | en |
dc.subject | Veterinary Sciences | en |
dc.title | Strain-Specific Sensitivity Estimates of Mycobacterium avium subsp paratuberculosis Culture in Greek Sheep and Goats | en |
dc.type | journalArticle | en |
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