dc.creator | Chondrogiannis, G. | en |
dc.creator | Kastamoulas, M. | en |
dc.creator | Kanavaros, P. | en |
dc.creator | Vartholomatos, G. | en |
dc.creator | Bai, M. | en |
dc.creator | Baltogiannis, D. | en |
dc.creator | Sofikitis, N. | en |
dc.creator | Arvanitis, D. | en |
dc.creator | Galani, V. | en |
dc.date.accessioned | 2015-11-23T10:24:39Z | |
dc.date.available | 2015-11-23T10:24:39Z | |
dc.date.issued | 2014 | |
dc.identifier | 10.1155/2014/536049 | |
dc.identifier.issn | 2314-6133 | |
dc.identifier.uri | http://hdl.handle.net/11615/26650 | |
dc.description.abstract | We analyzed the effects of IL-13, IFN-gamma, and IL-1 beta on cell viability and death of LNCaP and PC-3 cells and major signaling pathways involved in these effects. Significant increase of LNCaP cell death (apoptotic and necrotic) and increased levels of active caspase 3 were observed in cells treated with inhibitors of ERK 1/2 (UO126) and p38 (SB203580) prior to IL-1 beta treatment in comparison to cells treated with UO126, SB203580, or IL-1 beta alone. Significant increase of LNCaP but not PC-3 cell death was detected after treatment with LY-294002 (inhibitor of phosphatidylinositol 3-kinase). No significant increase of LNCaP and PC-3 cell death was observed after treatment with SP600125 (inhibitor of JNK), SB203580 (inhibitor of p38), UO126 (inhibitor of ERK 1/2), or BAY 11-7082 (inhibitor of NF-kappa B). Reduced c-FLIPL expression was observed in LNCaP cells treated with LY-294002. The significant potentiation of LNCaP cell death by inhibition of ERK 1/2, p38, and PI3-K pathways may provide a rationale for therapeutic approach in androgen-dependent prostate cancer. | en |
dc.source | Biomed Research International | en |
dc.source.uri | <Go to ISI>://WOS:000337468900001 | |
dc.subject | NF-KAPPA-B | en |
dc.subject | PRO-APOPTOTIC ROLE | en |
dc.subject | CANCER CELLS | en |
dc.subject | MEDIATED APOPTOSIS | en |
dc.subject | TNF-ALPHA | en |
dc.subject | CONSTITUTIVE ACTIVATION | en |
dc.subject | SIGNALING PATHWAYS | en |
dc.subject | PI3K/AKT | en |
dc.subject | PATHWAY | en |
dc.subject | LNCAP CELLS | en |
dc.subject | EXPRESSION | en |
dc.subject | Biotechnology & Applied Microbiology | en |
dc.subject | Medicine, Research & Experimental | en |
dc.title | Cytokine Effects on Cell Viability and Death of Prostate Carcinoma Cells | en |
dc.type | journalArticle | en |