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Predominantly post-transcriptional regulation of activation molecules in chronic lymphocytic leukemia: The case of transferrin receptors
dc.creator | Chiotoglou, I. | en |
dc.creator | Smilevska, T. | en |
dc.creator | Samara, M. | en |
dc.creator | Likousi, S. | en |
dc.creator | Belessi, C. | en |
dc.creator | Athanasiadou, I. | en |
dc.creator | Stavroyianni, N. | en |
dc.creator | Samara, S. | en |
dc.creator | Laoutaris, N. | en |
dc.creator | Vamvakopoulos, N. | en |
dc.creator | Anagnostopoulos, A. | en |
dc.creator | Fassas, A. | en |
dc.creator | Stamatopoulos, K. | en |
dc.creator | Kollia, P. | en |
dc.date.accessioned | 2015-11-23T10:24:38Z | |
dc.date.available | 2015-11-23T10:24:38Z | |
dc.date.issued | 2008 | |
dc.identifier | 10.1016/j.bcmd.2008.05.003 | |
dc.identifier.issn | 1079-9796 | |
dc.identifier.uri | http://hdl.handle.net/11615/26645 | |
dc.description.abstract | Transcriptional and post-transcriptional control mechanisms have a differential impact on cellular physiology depending on activation status. Several lines of evidence suggest that chronic lymphocytic leukemia (CLL) malignant B cells resemble antigen-experienced and activated B cells. In the present study, we investigated the expression of transferrin receptor 1 (TfR1, CD71), one of the "classical" markers up-regulated upon B-cell activation, and TfR2, a novel receptor for transferrin, in peripheral blood CD19(+) B cells from tell healthy individuals and 76 patients with CLL so as to gain insight into potential disease-related differences in underlying regulatory mechanisms. Marked differences in the production and expression of these receptors were detected in malignant but not in normal B cells. Specifically, TfR1 mRNA and protein levels were significantly higher in comparison to TfR2, both in normal and malignant B cells. Furthermore, discrepancies between TfR mRNA and protein expression were observed in CLL; in contrast, mRNA and protein expression levels were generally concordant in normal B cells. Exposure to actinomycin D decreased TfR1 and TfR2 mRNA levels in normal CD19(+) B cells but had no effect on CLL malignant cells. The protein synthesis inhibitor cycloheximide had opposing effects in normal vs. CLL malignant B cells: thus, TfR1 and TfR2 mRNA levels were increased in normal B cells, whereas they were unaffected or even suppressed in CLL malignant B cells. These results allude to differential regulation of TfR1 and TfR2 expression in normal B cells vs. CLL. In normal B cells, transcriptional mechanisms exert a critical control over TfR1 and TfR2 expression, whereas in CLL post-transcriptional mechanisms seem to play a complementary and perhaps more important role. This type of control appears to be especially suited for modulation of genies implicated in proliferation of activated cells, like CLL malignant B cells. (C) 2008 Published by Elsevier Inc. | en |
dc.source | Blood Cells Molecules and Diseases | en |
dc.source.uri | <Go to ISI>://WOS:000258543000014 | |
dc.subject | transferrin receptor | en |
dc.subject | transcription | en |
dc.subject | B cell | en |
dc.subject | CLL | en |
dc.subject | NITRIC-OXIDE SYNTHASE | en |
dc.subject | B-CELL | en |
dc.subject | CD38 EXPRESSION | en |
dc.subject | GENE-EXPRESSION | en |
dc.subject | MUTATION STATUS | en |
dc.subject | IRON | en |
dc.subject | MECHANISMS | en |
dc.subject | IMMUNOGLOBULIN | en |
dc.subject | METABOLISM | en |
dc.subject | PHENOTYPE | en |
dc.subject | Hematology | en |
dc.title | Predominantly post-transcriptional regulation of activation molecules in chronic lymphocytic leukemia: The case of transferrin receptors | en |
dc.type | journalArticle | en |
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