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fbl Gene as a Species-Specific Target for Staphylococcus lugdunensis Identification
dc.creator | Chatzigeorgiou, K. S. | en |
dc.creator | Siafakas, N. | en |
dc.creator | Petinaki, E. | en |
dc.creator | Zerva, L. | en |
dc.date.accessioned | 2015-11-23T10:24:33Z | |
dc.date.available | 2015-11-23T10:24:33Z | |
dc.date.issued | 2010 | |
dc.identifier | 10.1002/jcla.20352 | |
dc.identifier.issn | 0887-8013 | |
dc.identifier.uri | http://hdl.handle.net/11615/26605 | |
dc.description.abstract | Staphylococcus lugdunensis is an unusually virulent coagulase-negative species, associated with severe infections The present report describes the development of a single-step, species-specific PCR protocol for S. lugdunensis identification fbl gene, encoding a fibrinogen-binding adhesin, was exploited and assessed as a suitable nucleic acid target The gene was detected in all 17 S lugdunensis isolates examined, while no amplification product was obtained from 98 isolates representing 11 staphylococcal and 17 nonstaphylococcal species Forty-seven percent of the S. lugdunensis strains produced a positive slide coagulase reaction, which is consistent with varying levels of Fbl protein expression within the species J. Clin Lab Anal 24:119-122, 2010. (C) 2010 Wiley-Liss, Inc | en |
dc.source | Journal of Clinical Laboratory Analysis | en |
dc.source.uri | <Go to ISI>://WOS:000276167700010 | |
dc.subject | fbl gene | en |
dc.subject | identification | en |
dc.subject | PCR | en |
dc.subject | Staphylococcus lugdunensis | en |
dc.subject | fbl protein | en |
dc.subject | expression | en |
dc.subject | POLYMERASE-CHAIN-REACTION | en |
dc.subject | LEVEL IDENTIFICATION | en |
dc.subject | TUF GENE | en |
dc.subject | STRAINS | en |
dc.subject | PROTEIN | en |
dc.subject | RNA | en |
dc.subject | DNA | en |
dc.subject | Medical Laboratory Technology | en |
dc.title | fbl Gene as a Species-Specific Target for Staphylococcus lugdunensis Identification | en |
dc.type | journalArticle | en |
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