dc.creator | Apostolakis, S. | en |
dc.creator | Vlata, Z. | en |
dc.creator | Vogiatzi, K. | en |
dc.creator | Krambovitis, E. | en |
dc.creator | Spandidos, D. A. | en |
dc.date.accessioned | 2015-11-23T10:22:37Z | |
dc.date.available | 2015-11-23T10:22:37Z | |
dc.date.issued | 2010 | |
dc.identifier | 10.1007/s11239-009-0424-1 | |
dc.identifier.issn | 0929-5305 | |
dc.identifier.uri | http://hdl.handle.net/11615/25708 | |
dc.description.abstract | The potential regulatory effect of angiotensins on circulating mononuclear cell activation and migration has not yet been thoroughly evaluated. Using flow cytometry we assessed the possible effect of angiotensin I and II on the expression of CX3CR1 and a single representative of each major chemokine family (CCR5 and CXCR4) in THP-1 monocytes, Jurcat T lymphocytes and primary monocytes-isolated from healthy donors. Fluorescence intensity and the rate of chemokine-positive cells was measured in na < ve cells and cells treated with angiotensin I and II. Neither angiotensin I nor angiotensin II exhibited any effect on fluorescence intensity and the rate of CX3CR1-, CCR5- and CXCR4-positive cells in primary peripheral blood mononuclear cells and Jurkat T cells. However, angiotensin II significantly increased the rate of CX3CR1-positive THP-1 cells. This effect was not attenuated by the pre-incubation of THP-1 cells with the AT-1 receptor blocker losartan, suggesting that this was not an AT-1-mediated effect. Angiotensin I and II had no effect on fluorescence intensity and the rate of CCR5- and CXCR4-positive THP-1 cells. In conclusion, angiotensin II increases the rate of CX3CR1-positive THP-1 cells. By extrapolating this in vitro observation to disease mechanisms, we speculate that angiotensin II induces up-regulation of CX3CR1 and promotes firm adhesion of circulation CX3CR1-positive monocytes on CX3CL1 expressing endothelial cells inducing vascular inflammation and atherogenesis. | en |
dc.source | Journal of Thrombosis and Thrombolysis | en |
dc.source.uri | <Go to ISI>://WOS:000277451600009 | |
dc.subject | Chemokine receptors | en |
dc.subject | CX3CR1 | en |
dc.subject | Angiotensin II | en |
dc.subject | Monocytes | en |
dc.subject | NF-KAPPA-B | en |
dc.subject | SMOOTH-MUSCLE | en |
dc.subject | T-CELLS | en |
dc.subject | RENIN | en |
dc.subject | ACTIVATION | en |
dc.subject | SYSTEM | en |
dc.subject | CHEMOKINES | en |
dc.subject | ATHEROSCLEROSIS | en |
dc.subject | HYPERTENSION | en |
dc.subject | RECEPTOR | en |
dc.subject | Hematology | en |
dc.subject | Peripheral Vascular Disease | en |
dc.title | Angiotensin II up-regulates CX3CR1 expression in THP-1 monocytes: impact on vascular inflammation and atherogenesis | en |
dc.type | journalArticle | en |