dc.creator | Alexopoulou, K. | en |
dc.creator | Foka, A. | en |
dc.creator | Petinaki, E. | en |
dc.creator | Jelastopulu, E. | en |
dc.creator | Dimitracopoulos, G. | en |
dc.creator | Spiliopoulou, I. | en |
dc.date.accessioned | 2015-11-23T10:22:01Z | |
dc.date.available | 2015-11-23T10:22:01Z | |
dc.date.issued | 2006 | |
dc.identifier | 10.1111/j.1472-765X.2006.01964.x | |
dc.identifier.issn | 0266-8254 | |
dc.identifier.uri | http://hdl.handle.net/11615/25472 | |
dc.description.abstract | Aims: Two commercial methods for the identification of coagulase-negative staphylococci (CNS) were compared with the restriction fragment length polymorphism (RFLP) of the amplified tuf gene, which served as the reference method. Methods and Results: One hundred and forty-five CNS were evaluated using the API 32 Staph ID and the Crystal GP/ID BBL systems. The PCR-RFLP of the tuf gene served as the reference method. The APIStaph and the GP/ID BBL had an overall rate of agreement with the molecular method of 58.6% and 46.2% respectively, with the inability of the GP/ID BBL to characterize 11.7% of the isolates. The APIStaph showed higher sensitivity and better agreement than the GP/ID BBL with the PCR-RFLP, except for Staphylococcus hominis and Staphylococcus capitis. Conclusions: Neither of the commercial systems was as reliable as the PCR-RFLP method for identifying isolates of CNS. Overall the APIStaph had better agreement with the PCR-RFLP than the GP/ID system. Significance and Impact of the Study: The results indicate that the PCR-RFLP method is more reliable than the two commercial systems tested, suggesting that it is more reliable for routinely identifying CNS. | en |
dc.source | Letters in Applied Microbiology | en |
dc.source.uri | <Go to ISI>://WOS:000240441400016 | |
dc.subject | APIStaph | en |
dc.subject | coagulase-negative staphylococci | en |
dc.subject | Crystal GP/ID | en |
dc.subject | identification | en |
dc.subject | PCR restriction fragment length polymorphism | en |
dc.subject | SPECIES-LEVEL IDENTIFICATION | en |
dc.subject | ACCURATE IDENTIFICATION | en |
dc.subject | SYSTEMS | en |
dc.subject | SCHEME | en |
dc.subject | Biotechnology & Applied Microbiology | en |
dc.subject | Microbiology | en |
dc.title | Comparison of two commercial methods with PCR restriction fragment length polymorphism of the tuf gene in the identification of coagulase-negative staphylococci | en |
dc.type | journalArticle | en |