Recherche
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Duplex Real-time PCR assay and SYBR green I melting curve analysis for molecular identification of HPV genotypes 16, 18, 31, 35, 51 and 66
(2015)
Long-term infection with high-risk HPV genotypes is the leading cause of cervical cancer. In the present study a Duplex Real-time PCR assay was developed in order to identify HPV types 16, 18, 31, 35, 51 and 66 in three ...
Optimization of TNF-alpha, overexpression in Escherichia coli using response surface methodology: Purification of the protein and oligomerization studies
(2012)
Tumor necrosis factor-alpha (TNF-alpha) is responsible for many autoimmune disorders including rheumatoid arthritis, psoriasis, Chron's disease, stroke, and atherosclerosis. Thus, inhibition of TNF-alpha is a major challenge ...
A statistical approach for optimization of RANKL overexpression in Escherichia coli: Purification and characterization of the protein
(2013)
Receptor activator of nuclear factor-kappa B (RANK) and its cognate ligand (RANICL) is a member of the TNF superfamily of cytokines which is essential in osteobiology and its overexpression has been implicated in the ...
Statistical approaches to maximize recombinant protein expression in Escherichia coli: A general review
(2014)
The supply of many valuable proteins that have potential clinical or industrial use is often limited by their low natural availability. With the modern advances in genomics, proteomics and bioinformatics, the number of ...
A new RT-PCR assay for the identification of the predominant recombination types in 2C and 3D genomic regions of vaccine-derived poliovirus strains
(2010)
In the post-eradication era of wild polioviruses, the only remaining sources of poliovirus infection worldwide would be the vaccine-derived polioviruses (VDPVs). As the preponderance of countries certified to be polio-free ...
Use of mutational pattern in 5 '-NCR and VP1 regions of polioviruses for molecular diagnosis
(2007)
Polioviruses are members of the enterovirus genus, belonging to the Picornaviridae family. They are the causative agents of poliomyelitis, a paralytic and sometimes fatal disease in humans. The number of poliomyelitis cases ...
Characterization of novel intergenogroup and intergenotype recombinant noroviruses from central Greece
(2014)
Noroviruses (NoVs) are a major causative agent of acute gastroenteritis in humans. They are members of the Caliciviridae family and based on the genetic analysis of the RdRp and capsid regions, human NoVs are divided into ...
Direct extraction and molecular characterization of enteroviruses genomes from human faecal samples
(2008)
Routine diagnosis of acute flaccid paralysis (AFP) is still based on classical virological procedures. Several enteroviruses serotypes are not easily isolated in cell cultures system used and routinely more than one passage ...
A comparative amplification of five different genomic regions on Coxsackie A and B viruses. Implications in clinical diagnostics
(2005)
Modern molecular approaches in Human Enterovirus detection rely on the designing of generic and often degenerate primers in order to amplify specific sequences within the enterovirus genome. In the present study a comparative ...
Molecular identification of enteroviruses responsible for an outbreak of aseptic meningitis; implications in clinical practice and epidemiology
(2004)
An outbreak of aseptic meningitis was recorded in Greece during the year 2001. Detection of the clinical strains was achieved by performing reverse transcription-polymerase chain reaction (RT-PCR) on RNA isolated from cell ...