Αναζήτηση
Αποτελέσματα 1-10 από 10
Variation in sulfation pattern containing proteoglycans development of uterine of galactosaminoglycan is associated with the leiomyoma
(2004)
The variations in sulfation patterns of glycosaminoglycans (GAGs) present in human normal myometrium, and uterine leiomyoma were characterized following specific enzymic treatments and analysis by high-performance capillary ...
Use of mutational pattern in 5 '-NCR and VP1 regions of polioviruses for molecular diagnosis
(2007)
Polioviruses are members of the enterovirus genus, belonging to the Picornaviridae family. They are the causative agents of poliomyelitis, a paralytic and sometimes fatal disease in humans. The number of poliomyelitis cases ...
Inhibitor design for ribonuclease A: the binding of two 5 '-phosphate uridine analogues
(2009)
In the quest for the rational design of selective and potent inhibitors for members of the pancreatic ribonuclease A (RNase A) family of biomedical interest, the binding of uridine 5'-phosphate (U5P) and uridine 5'-diphosphate ...
In vivo evaluation of CYP1A2 CYP2A6, NAT-2 and xanthine oxidase activities in a Greek population sample by the RP-HPLC monitoring of caffeine metabolic ratios
(2007)
A RP-HPLC method was developed for the assessment of caffeine and its metabolites in urine and was used for the evaluation of the CYP1A2, CYP2A6, xanthine oxidase (XO) and N-acetyl-transferase-2 (NAT-2) in vivo activities ...
Direct extraction and molecular characterization of enteroviruses genomes from human faecal samples
(2008)
Routine diagnosis of acute flaccid paralysis (AFP) is still based on classical virological procedures. Several enteroviruses serotypes are not easily isolated in cell cultures system used and routinely more than one passage ...
A comparative amplification of five different genomic regions on Coxsackie A and B viruses. Implications in clinical diagnostics
(2005)
Modern molecular approaches in Human Enterovirus detection rely on the designing of generic and often degenerate primers in order to amplify specific sequences within the enterovirus genome. In the present study a comparative ...
Identification of the commonest cystic fibrosis transmembrane regulator gene Delta F508 mutation: evaluation of PCR-single-strand conformational polymorphism and polyacrylamide gel electrophoresis
(2006)
In the present study we investigated whether single-strand conformational polymorphism (SSCP) and polyacrylamide gel electrophoresis (PAGE) could be used for the identification of the CFTR Delta F508 gene mutation, which ...
Molecular identification of enteroviruses responsible for an outbreak of aseptic meningitis; implications in clinical practice and epidemiology
(2004)
An outbreak of aseptic meningitis was recorded in Greece during the year 2001. Detection of the clinical strains was achieved by performing reverse transcription-polymerase chain reaction (RT-PCR) on RNA isolated from cell ...
Amplification of Echoviruses genomic regions by different RT-PCR protocols - a comparative study
(2004)
In the present report, the results of a comparative study in the detection of all Echoviruses reference strains as well as of 38 clinical isolates are presented. Using RT-PCR with already published primer pairs (UG(52)-UC53, ...
High sequence divergence in the 5 ' non-coding region of reference Coxsackie B and ECHO viral strains and clinical isolates revealed by restriction fragment length polymorphism analysis
(2001)
We report the restriction fragment length polymorphism (RFLP) patterns of a 440-bp-long 5' non-coding region (5'NCR) amplification target of all 34 reference Coxsackie B and ECHO (enteric cytopathic human orphan) enterovirus ...