Browsing by Subject "polymerase chain reaction"

Acinetobacter baumannii antibiotic resistance mechanisms

Kyriakidis I., Vasileiou E., Pana Z.D., Tragiannidis A. (2021)

Acinetobacter baumannii is a Gram-negative ESKAPE microorganism that poses a threat to public health by causing severe and invasive (mostly nosocomial) infections linked with high mortality rates. During the last years, ...

An Activating Janus kinase-3 mutation is associated with cytotoxic T lymphocyte antigen-4-dependent Immune dysregulation syndrome

Sic H., Speletas M., Cornacchione V., Seidl M., Beibel M., Linghu B., Yang F., Sevdali E., Germenis A.E., Oakeley E.J., Vangrevelinghe E., Sailer A.W., Traggiai E., Gram H., Eibel H. (2017)

Heterozygous mutations in the cytotoxic T lymphocyte antigen-4 (CTLA-4) are associated with lymphadenopathy, autoimmunity, immune dysregulation, and hypogammaglobulinemia in about 70% of the carriers. So far, the incomplete ...

Acute non-A, non-B, non-C hepatitis differences and similarities between hepatitis E virus infection and autoimmune hepatitis, with phylogenetic analysis of hepatitis E virus in humans and wild boars

Zachou K., Azariadis K., Sofia M., Lyberopoulou A., Arvaniti P., Gatselis N., Spyrou V., Billinis C., Dalekos G.N. (2022)

Background Hepatitis E virus (HEV) infection incidence is increasing in Europe, accounting for the majority of acute hepatitis cases. We investigated the prevalence and clinical characteristics of acute hepatitis E (AHE) ...

Aldehyde dehydrogenase 1B1: Molecular cloning and characterization of a novel mitochondrial acetaldehyde-metabolizing enzyme

Stagos, D.; Chen, Y.; Brocker, C.; Donald, E.; Jackson, B. C.; Orlicky, D. J.; Thompson, D. C.; Vasiliou, V. (2010)

Ethanol-induced damage is largely attributed to its toxic metabolite, acetaldehyde. Clearance of acetaldehyde is achieved by its oxidation, primarily catalyzed by the mitochondrial class II aldehyde dehydrogenase (ALDH2). ...

Antibiotic Resistance Patterns and mcr-1 Detection in Avian Pathogenic Escherichia coli Isolates from Commercial Layer and Layer Breeder Flocks Demonstrating Colibacillosis in Greece

Koutsianos D., Athanasiou L.V., Dimitriou T., Nikolaidis M., Tsadila C., Amoutzias G., Mossialos D., Koutoulis K.C. (2021)

Objectives: The aim of this study was to investigate the antimicrobial resistance (AMR) patterns of Escherichia coli strains isolated from poultry flocks suffering from colibacillosis in Greece and to detect the presence ...

The antiphospholipid syndrome and infection

Dalekos, G. N.; Zachou, K.; Liaskos, C. (2001)

Infectious agents have been implicated in the induction of antiphospholipid (aPL) antibodies and the development of the antiphospholipid syndrome (APS). This review focuses on the types of aPL antibodies detected in ...

Application of Bayesian modeling for diagnostic assays of Mycobacterium avium subsp. paratuberculosis in sheep and goats flocks

Hemati Z., Meletis E., Derakhshandeh A., Haghkhah M., Kostoulas P., Singh S.V., Chaubey K.K., Gupta S. (2022)

Background: This study aimed to screen the sera of goats and sheep from flocks suspected of Mycobacterium avium subsp. paratuberculosis (MAP) infection by a newly standardized Mce-truncated ELISA (Mt-ELISA) kit for the ...

Application of fingerprinting molecular methods in bioremediation studies

Karpouzas, D. G.; Singh, B. K. (2010)

Bioremediation has been identified as a beneficial and effective strategy for the removal of recalcitrant environmental contaminants. Bioaugmentation of polluted environments with exogenous degrading microorganisms constitutes ...

Assessment of the effects of oxamyl on the bacterial community of an agricultural soil exhibiting enhanced biodegradation

Gallego S., Devers-Lamrani M., Rousidou K., Karpouzas D.G., Martin-Laurent F. (2019)

Modern agricultural practices largely rely on pesticides to protect crops against various pests and to ensure high yields. Following their application to crops a large amount of pesticides ends up in soil where they may ...

Assessment of the impact of three pesticides on microbial dynamics and functions in a lab-to-field experimental approach

Karas P.A., Baguelin C., Pertile G., Papadopoulou E.S., Nikolaki S., Storck V., Ferrari F., Trevisan M., Ferrarini A., Fornasier F., Vasileiadis S., Tsiamis G., Martin-Laurent F., Karpouzas D.G. (2018)

The toxicity of pesticides on soil microorganisms is as an emerging area of concern. Novel and well-standardized tools could be now used to provide a robust assessment of the ecotoxicity of pesticides on soil microorganisms. ...

Association of anti-CCP positivity and carriage of TNFRII susceptibility variant with anti-TNF-α response in rheumatoid arthritis

Vasilopoulos, Y.; Bagiatis, V.; Stamatopoulou, D.; Zisopoulos, D.; Alexiou, I.; Sarafidou, T.; Settas, L.; Sakkas, L.; Mamuris, Z. (2011)

Objective: To investigate the possible influence of tumour necrosis factoralpha (TNF), TNF receptor I (TNFRI) and TNF receptor II (TNFRII) gene polymorphisms on anti-TNF treatment responsiveness, stratified by autoantibody ...

Association of codon 72 polymorphism of p53 with the severity of cervical dysplasia, E6-T350G and HPV16 variant lineages in HPV16-infected women

Moschonas G.D., Tsakogiannis D., Lamprou K.A., Mastora E., Dimitriou T.G., Kyriakopoulou Z., Kottaridi C., Karakitsos P., Markoulatos P. (2017)

Purpose. Polymorphic variability in the tumour-suppressor protein p53 at codon 72 has a considerable impact on cervical cancer development. The present study clarified the association between p53 codon 72 genotypes and the ...

Association of p16 (CDKN2A) polymorphisms with the development of HPV16-related precancerous lesions and cervical cancer in the Greek population

Tsakogiannis D., Moschonas G.D., Bella E., Kyriakopoulou Z., Amoutzias G.D., Dimitriou T.G., Kottaridi C., Markoulatos P. (2018)

The tumor suppressor protein p16 plays a fundamental role in cell cycle regulation and exerts a protective effect against tumor growth. Two different polymorphisms at positions 540 and 580 at the 3′UTR of exon 3 of p16 ...

Autoimmune hepatitis type-2 and Epstein-Barr virus infection in a toddler: Art of facts or an artifact?

Zellos, A.; Spoulou, V.; Roma-Giannikou, E.; Karentzou, O.; Dalekos, G. N.; Theodoridou, M. (2013)

Epstein-Barr virus (EBV) can cause frequently asymptomatic (or anicteric) and self-limited hepatitis, while occasionally may result in considerable cholestatic hepatitis. Herein, we describe the case of a previously healthy ...

Bayesian estimation of sensitivity and specificity of a PCR method to detect Coxiella burnetii in milk and vaginal secretions in sheep and goat samples

Abiri Z., Khalili M., Kostoulas P., Sharifi H., Rad M., Babaei H. (2019)

Coxiella burnetii is a gram-negative and polymorphic rod bacterium that causes Q fever, a common zoonotic disease distributed worldwide. Widespread occurrences of the disease outbreaks indicate the importance of coordinated ...

Bilateral Pleural Effusion in Rheumatoid Arthritis: Think Beyond the Obvious

Dimeas I.E., Sinis S.I., Dimeas G.E., Daniil Z. (2022)

An 86-year-old woman, non-smoker, with a medical history of rheumatoid arthritis for 20 years (stable at 5mg of prednisolone) was referred from a peripheral hospital to the Department of Respiratory Medicine of our tertiary ...

Bing–Neel syndrome presenting with bilateral lumbar radiculopathy: A case report of a rare clinical entity [Síndrome de Bing-Neel que se presenta con radiculopatía lumbar bilateral: reporte de caso de una entidad clínica rara]

Brotis A.G., Palassopoulou M., Kapsalaki E.Z., Paschalis T., Papastergiou V., Fountas K.N. (2022)

The differential diagnosis of bilateral lower extremity weakness is broad. We present a very rare case of a 48-year old male patient, with walking difficulties due to Bing–Neel syndrome. On clinical examination, there was ...

Cell-specific and roasting-dependent regulation of the Keap1/Nrf2 pathway by coffee extracts

Priftis A., Angeli-Terzidou A.-E., Veskoukis A.S., Spandidos D.A., Kouretas D. (2018)

Coffee is a popular beverage that contains various bioactive compounds. However, its molecular mechanism of action is not fully elucidated. In this context, two previously characterized coffee extracts, a lightly roasted ...

Characterisation of a ST100 Staphylococcus epidermidis producing an LnuB nucleotidyltransferase: Evidence for interspecies spread of an lnuB-carrying transposon

Papagiannitsis C.C., Sarrou S., Tsilipounidaki K., Malli E., Medvecky M., Hrabak J., Fthenakis G.C., Petinaki E. (2018)

[No abstract available]

Clear detection and typing of Herpes Simplex Virus types 1 and 2 by an indirect ELISA assay: Comparison with three different combined methods - Capture ELISA, restriction enzymes, and polymerase chain reaction

Markoulatos, P.; Fountoucidou, P.; Marinakis, G.; Krikelis, V.; Spyrou, N.; Vamvakopoulos, N.; Moncany, M. L. J. (1997)

The severity and recurrences of Herpes Simplex Virus (HSV) infection depend on the type of the infectious agent (HSV-1 or HSV-2), which induces the necessity of a nonambiguous detecting typing. The commonly used capture ...