Πλοήγηση ανά Θέμα "POLYMERASE-CHAIN-REACTION"
Αποτελέσματα 1-20 από 30
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Advances in the pathogenesis of canine leishmaniosis: epidemiologic and diagnostic implications
(2009)Canine leishmaniosis caused by Leishmania infantum (Syn. L. chagasi) is an important zoonosis with a complex pathogenesis. Parasite transmission occurs via female sandflies that inject promastigotes into the skin of the ... -
Atypical presentation of varicella.-zoster virus encephalitis in an immunocompetent adult
(2008)BACKGROUND: Varicella-zoster virus encephalitis is uncommon, but not rare, in immunocompetent adults. Typically, patients develop stroke with hemiplegia caused by large vessel vasculopathy days to weeks after herpes zoster ... -
A clinical case of chicken infectious anemia disease and virus DNA detection in naturally infected broilers in Greece
(2007)In this study, chicken infectious anemia virus (CIAV) DNA was detected from 12-day-old broilers. Clinical history showed that the clinical features were diarrhea, blue wing disease, depression, and death. Necropsy findings ... -
A comparative amplification of five different genomic regions on Coxsackie A and B viruses. Implications in clinical diagnostics
(2005)Modern molecular approaches in Human Enterovirus detection rely on the designing of generic and often degenerate primers in order to amplify specific sequences within the enterovirus genome. In the present study a comparative ... -
Cytological and molecular detection of Leishmania infantum in different tissues of clinically normal and sick cats
(2014)Natural infection of domestic cats by Leishmania infantum (synonym: L chagasi) has been demonstrated in several European, Latin American, and Asian countries, and the estimated prevalence of infection, based mainly on blood ... -
Development of a two-step high-resolution melting (HRM) analysis for screening sequence variants associated with resistance to the QoIs, benzimidazoles and dicarboximides in airborne inoculum of Botrytis cinerea
(2014)A rapid, high-resolution melting (HRM) analysis protocol was developed to detect sequence variations associated with resistance to the QoIs, benzimidazoles and dicarboximides in Botrytis cinerea airborne inoculum. HRM ... -
Diagnostic performance of a rapid in-clinic test for the detection of Canine Parvovirus under different storage conditions and vaccination status
(2015)Canine parvovirus (CPV) is one of the most common causes of acute haemorrhagic enteritis in young dogs, while clinical diagnosis is often indecisive. The aim of our study was to evaluate the diagnostic accuracy of an ... -
Direct extraction and molecular characterization of enteroviruses genomes from human faecal samples
(2008)Routine diagnosis of acute flaccid paralysis (AFP) is still based on classical virological procedures. Several enteroviruses serotypes are not easily isolated in cell cultures system used and routinely more than one passage ... -
Early diagnosis of enteromyxosis in intensively reared sharpsnout seabream, Diplodus puntazzo
(2014)Myxozoans are metazoan parasites which are highly pathogenic in commercially important fish, particularly in aquaculture. Enteromyxosis is an economically significant disease caused by the myxosporean parasite Enteromyxum ... -
Effect of origanum vulgaris and allium sativum extracts for the control of proliferative enteropathy in weaning pigs
(2009)The aim of the present trial was to investigate the efficacy of Virbamix PE (R) (Virbac SA, France) an appetite enhancer and feed flavouring material containing plant extracts of Origanum vulgaris and Allium sativum, added ... -
Epidemiological study of Q fever in humans, ruminant animals, and ticks in Cyprus using a geographical information system
(2006)A cross-sectional study of Q fever was conducted in a representative sample of the human and animal population in Cyprus in order to assess the seroprevalence of Q fever and the prevalence of related risk factors. A total ... -
Evaluation of lymph node and bone marrow cytology in the diagnosis of canine leishmaniasis (Leishmania infantum) in symptomatic and asymptomatic
(2005)The sensitivity and specificity of lymph node and bone marrow smear microscopy for the diagnosis of Leishmania infantum-infected dogs was evaluated in 79 dogs with leishmaniasis (Group A), 52 asymptomatically infected dos ... -
Evaluation of seroneutralization and molecular diagnostic methods for echovirus identification
(2005)In this study we compared the identification results of 41 echovirus clinical isolates using RIVM pools (National Institute for Public Health and the Environment RIVM, Bilthoven, The Netherlands) and reverse transcriptio ... -
Fate of enterotoxigenic Staphylococcus aureus and staphylococcal enterotoxins in Feta and Galotyri cheeses
(2012)In this study the fate of enterotoxigenic Staphylococcus aureus and staphylococcal enterotoxins in Feta and Galotyri cheeses were studied. Initially, the enterotoxigenic abilities of four Staph. aureus LHA, LHB, LHC and ... -
fbl Gene as a Species-Specific Target for Staphylococcus lugdunensis Identification
(2010)Staphylococcus lugdunensis is an unusually virulent coagulase-negative species, associated with severe infections The present report describes the development of a single-step, species-specific PCR protocol for S. lugdunensis ... -
High-resolution melting analysis for rapid detection and characterization of Botrytis cinerea phenotypes resistant to fenhexamid and boscalid
(2014)A novel, high-resolution melting (HRM) analysis was developed to detect single nucleotide polymorphisms (SNPs) associated with resistance to fenhexamid (hydroxyanilides) and boscalid (succinate dehydrogenase inhibitors) ... -
Isolation of Mycoplasma bovis from bovine clinical mastitis cases in Northern Greece
(2007)Mycoplasnia bovis was detected in 18/219 (8.2%) quarter milk samples collected from cases of bovine clinical mastitis in Northern Greece between November 1997 and March 1999. The cases occurred in 2/37 (5.4%) of the herds ... -
Laboratory diagnosis of contagious ecthyma: Comparison of different PCR protocols with virus isolation in cell culture
(2006)A new polymerase chain reaction (PCR) assay for rapid diagnosis of contagious ecthyma was designed and applied to 21 clinical samples from Greece. This assay, which detects a highly conserved gene from the parapox genome, ... -
A large waterborne gastroenteritis outbreak in central Greece, March 2012: challenges for the investigation and management
(2014)In March 2012, there was an unusual increase of gastroenteritis cases in a district with 37264 inhabitants in central Greece. It was estimated that more than 3600 people developed symptoms. A 1:1 case-control study showed ... -
Maize Authentication: Quality Control Methods and Multivariate Analysis (Chemometrics)
(2009)Maize is one of the most important cereals because of its numerous applications in processed foods where it is the major or minor component. Apart from maize authenticity issues related to cultivar and geographical origin ...