Πλοήγηση ανά Συγγραφέα "Markoulatos, P."
Αποτελέσματα 1-20 από 66
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Amplification of Echoviruses genomic regions by different RT-PCR protocols - a comparative study
(2004)In the present report, the results of a comparative study in the detection of all Echoviruses reference strains as well as of 38 clinical isolates are presented. Using RT-PCR with already published primer pairs (UG(52)-UC53, ... -
Characterization of novel intergenogroup and intergenotype recombinant noroviruses from central Greece
(2014)Noroviruses (NoVs) are a major causative agent of acute gastroenteritis in humans. They are members of the Caliciviridae family and based on the genetic analysis of the RdRp and capsid regions, human NoVs are divided into ... -
Circulation of intergenotype recombinant noroviruses GII.9/GII.6 from 2006 to 2011 in central Greece
(2014)Noroviruses (NoVs) are members of the Caliciviridae family and are recognized as a worldwide cause of acute nonbacterial gastroenteritis. Based on the genetic analysis of the RdRp and capsid regions, human NoVs are divided ... -
Classification and structure of echovirus 5 '-UTR sequences
(2005)Enteroviruses are classified into two genetic clusters on the basis of 5'-UTR and all echoviruses (ECV) are classified together with coxsackie B viruses (CBV), coxsackie A viruses (CAV) types 2-10, 12, 14 and 16, and ... -
Clear detection and typing of Herpes Simplex Virus types 1 and 2 by an indirect ELISA assay: Comparison with three different combined methods - Capture ELISA, restriction enzymes, and polymerase chain reaction
(1997)The severity and recurrences of Herpes Simplex Virus (HSV) infection depend on the type of the infectious agent (HSV-1 or HSV-2), which induces the necessity of a nonambiguous detecting typing. The commonly used capture ... -
Combined 5 ' UTR RFLP analysis and VP1 sequencing for epidemic investigation of enteroviruses
(2013)Enteroviruses, the main cause of aseptic meningitis, consist of 100 serotypes, and many of them have been associated with large outbreaks. In the present study, a comparison of RFLP analysis of the 5' untranslated region ... -
A comparative amplification of five different genomic regions on Coxsackie A and B viruses. Implications in clinical diagnostics
(2005)Modern molecular approaches in Human Enterovirus detection rely on the designing of generic and often degenerate primers in order to amplify specific sequences within the enterovirus genome. In the present study a comparative ... -
A comparative study of immunocapture ELISA and RT-PCR for screening clinical samples from Southern Greece for human influenza virus types A and B
(2000)An immunocapture (IC) ELISA and reverse transcriptase (RT)-PCR assays were evaluated as screening methods for the detection of influenza virus types A and B in clinical samples collected from individuals presenting with ... -
Complete genomic characterization of an intertypic Sabin 3/Sabin 2 capsid recombinant
(2008)The genetic properties of strain K/2002, isolated from fecal samples of a 7-month-old child who had received his first oral poliovirus vaccine (OPV) dose at the age of 3 months, are described. Preliminary sequencing ... -
Complete nucleotide sequence analysis of the VP1 genomic region of Echoviruses 6 isolated from sewage in Greece revealed 98% similarity with Echoviruses 6 that were characterized from an aseptic meningitis outbreak 1 year later
(2011)The molecular characterization of two enterovirus strains (LR51A5 and LR61G3) isolated from the sewage treatment plant unit in Larissa, Greece, in May and June 2006 and the investigation of their relationship with enteroviruses ... -
Concentration, Detection and Identification of Infectious Enteroviruses in Sewage
(2008)This work presents a new approach for the molecular 'serotyping' of enteroviruses concentrated and isolated from sewage. Viruses were adsorbed to cellulose nitrate membrane filters, isolated from the membrane filter using ... -
Correlation between recombination junctions and RNA secondary structure elements in poliovirus Sabin strains
(2010)In order to test the hypothesis that RNA structural elements promote the distribution of certain types of recombination junctions in each one of the 2C and 3D poliovirus genomic regions (Sabin 3/Sabin 2 or Sabin 1 in 2C ... -
Correlation of mutations and recombination with growth kinetics of poliovirus vaccine strains
(2010)Attenuated strains of Sabin poliovirus vaccine replicate in the human gut and, in rare cases, may cause vaccine-associated paralytic poliomyelitis (VAPP). The genetic instability of Sabin strains constitutes one of the ... -
Detection of unusual mutation within the VP1 region of different re-isolates of poliovirus Sabin vaccine
(2006)In the present study, a genomic analysis of full VP1 sequence region of 15 clinical re-isolates (14 healthy vaccinees and one bone marrow tumor patient) was conducted, aiming to the identification of mutations and to the ... -
Determination of antigenic properties of vaccine derived poliovirus strains
(2010)In this study, the immunity level of the southern Greek population in the 1-10-year, 11-20-year, 21-30-year and 31-40-year age groups with regard to Sabin vaccine strains and a collection of 11 recombinant and three ... -
Determination of human papillomavirus 16 physical status through E1/E6 and E2/E6 ratio analysis
(2014)Human papillomavirus (HPV) 16 genome integration into the host chromosome is a crucial event during the life cycle of the virus and a major step towards carcinogenesis. The integration of HPV16 DNA promotes a constitutive ... -
Development of a single-tube polymerase chain reaction assay for the simultaneous detection of Haemophilus influenzae, Pseudomonas aeruginosa, Staphylococcus aureus, and Streptococcus spp. directly in clinical samples
(2009)This study describes the development and evaluation of a multiplex single-tube polymerase chain reaction assay for the simultaneous detection of Haemophilus influenzae, Pseudomonas aeruginosa, Staphylococcus aureus, and ... -
Direct extraction and molecular characterization of enteroviruses genomes from human faecal samples
(2008)Routine diagnosis of acute flaccid paralysis (AFP) is still based on classical virological procedures. Several enteroviruses serotypes are not easily isolated in cell cultures system used and routinely more than one passage ... -
Duplex Real-time PCR assay and SYBR green I melting curve analysis for molecular identification of HPV genotypes 16, 18, 31, 35, 51 and 66
(2015)Long-term infection with high-risk HPV genotypes is the leading cause of cervical cancer. In the present study a Duplex Real-time PCR assay was developed in order to identify HPV types 16, 18, 31, 35, 51 and 66 in three ... -
Endocytosis of hepatitis C virus non-enveloped capsid-like particles induces MAPK-ERK1/2 signaling events
(2010)Although HCV is an enveloped virus, naked nucleocapsids have been reported in the serum of infected patients. The HCV core particle serves as a protective capsid shell for the viral genome and recombinant in vitro assembled ...