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Optimization of TNF-alpha, overexpression in Escherichia coli using response surface methodology: Purification of the protein and oligomerization studies
(2012)Tumor necrosis factor-alpha (TNF-alpha) is responsible for many autoimmune disorders including rheumatoid arthritis, psoriasis, Chron's disease, stroke, and atherosclerosis. Thus, inhibition of TNF-alpha is a major challenge ...