Sfoglia per Autore "Papaneophytou, C. P."

Dietary Supplementation of Benzoic Acid and Essential Oil Compounds Affects Buffering Capacity of the Feeds, Performance of Turkey Poults and Their Antioxidant Status, pH in the Digestive Tract, Intestinal Microbiota and Morphology

Giannenas, I.; Papaneophytou, C. P.; Tsalie, E.; Pappas, I.; Triantafillou, E.; Tontis, D.; Kontopidis, G. A. (2014)

Three trials were conducted to evaluate the effect of supplementation of a basal diet with benzoic acid or thymol or a mixture of essential oil blends (MEO) or a combination of benzoic acid with MEO (BMEO) on growth ...

The effects of benzoic acid and essential oil compounds in combination with protease on the performance of chickens

Giannenas, I. A.; Papaneophytou, C. P.; Tsalie, E.; Triantafillou, E.; Tontis, D.; Kontopidis, G. A. (2014)

Experiments were conducted to study the effect of benzoic acid and of essential oil blends in combination with protease on the growth performance of broiler chickens. In the first trial, the birds were divided into three ...

Optimization of TNF-alpha, overexpression in Escherichia coli using response surface methodology: Purification of the protein and oligomerization studies

Papaneophytou, C. P.; Kontopidis, G. A. (2012)

Tumor necrosis factor-alpha (TNF-alpha) is responsible for many autoimmune disorders including rheumatoid arthritis, psoriasis, Chron's disease, stroke, and atherosclerosis. Thus, inhibition of TNF-alpha is a major challenge ...

Quantification of the Effects of Ionic Strength, Viscosity, and Hydrophobicity on Protein-Ligand Binding Affinity

Papaneophytou, C. P.; Grigoroudis, A. I.; McInnes, C.; Kontopidis, G. (2014)

In order to quantify the interactions between molecules of biological interest, the determination of the dissociation constant (K-d) is essential. Estimation of the binding affinity in this way is routinely performed in ...

Rationally Designed Less Toxic SPD-304 Analogs and Preliminary Evaluation of Their TNF Inhibitory Effects

Alexiou, P.; Papakyriakou, A.; Ntougkos, E.; Papaneophytou, C. P.; Liepouri, F.; Mettou, A.; Katsoulis, I.; Maranti, A.; Tsiliouka, K.; Strongilos, A.; Chaitidou, S.; Douni, E.; Kontopidis, G.; Kollias, G.; Couladouros, E.; Eliopoulos, E. (2014)

SPD-304 was discovered as a promising tumor necrosis factor alpha (TNF) antagonist that promotes dissociation of TNF trimers and therefore blocks the interaction of TNF and its receptor. However, SPD-304 contains a potentially ...

Solvent Selection for Insoluble Ligands, a Challenge for Biological Assay Development: A TNF-alpha/SPD304 Study

Papaneophytou, C. P.; Mettou, A. K.; Rinotas, V.; Douni, E.; Kontopidis, G. A. (2013)

Many active compounds may be excluded from biological assays due to their low aqueous solubility. In this study, a simple method for the determination of the solubility of compounds containing aromatic rings is proposed. ...

A statistical approach for optimization of RANKL overexpression in Escherichia coli: Purification and characterization of the protein

Papaneophytou, C. P.; Rinotas, V.; Douni, E.; Kontopidis, G. (2013)

Receptor activator of nuclear factor-kappa B (RANK) and its cognate ligand (RANICL) is a member of the TNF superfamily of cytokines which is essential in osteobiology and its overexpression has been implicated in the ...

Statistical approaches to maximize recombinant protein expression in Escherichia coli: A general review

Papaneophytou, C. P.; Kontopidis, G. (2014)

The supply of many valuable proteins that have potential clinical or industrial use is often limited by their low natural availability. With the modern advances in genomics, proteomics and bioinformatics, the number of ...