Sfoglia per Autore "Levidiotou-Stefanou, S."
Items 1-20 di 22
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Characterization of novel intergenogroup and intergenotype recombinant noroviruses from central Greece
Ruether, I. G. A.; Dimitriou, T. G.; Tsakogiannis, D.; Kyriakopoulou, Z.; Amoutzias, G. D.; Gartzonika, C.; Levidiotou-Stefanou, S.; Markoulatos, P. (2014)Noroviruses (NoVs) are a major causative agent of acute gastroenteritis in humans. They are members of the Caliciviridae family and based on the genetic analysis of the RdRp and capsid regions, human NoVs are divided into ... -
Classification and structure of echovirus 5 '-UTR sequences
Siafakas, N.; Papaventsis, D.; Levidiotou-Stefanou, S.; Vamvakopoulos, N. C.; Markoulatos, P. (2005)Enteroviruses are classified into two genetic clusters on the basis of 5'-UTR and all echoviruses (ECV) are classified together with coxsackie B viruses (CBV), coxsackie A viruses (CAV) types 2-10, 12, 14 and 16, and ... -
Combined 5 ' UTR RFLP analysis and VP1 sequencing for epidemic investigation of enteroviruses
Kyriakopoulou, Z.; Tsolis, K.; Pliaka, V.; Tsakogiannis, D.; Ruether, I. G. A.; Gartzonika, C.; Levidiotou-Stefanou, S.; Markoulatos, P. (2013)Enteroviruses, the main cause of aseptic meningitis, consist of 100 serotypes, and many of them have been associated with large outbreaks. In the present study, a comparison of RFLP analysis of the 5' untranslated region ... -
Complete genomic characterization of an intertypic Sabin 3/Sabin 2 capsid recombinant
Dedepsidis, E.; Pliaka, V.; Kyriakopoulou, Z.; Brakoulias, C.; Levidiotou-Stefanou, S.; Pratti, A.; Mamuris, Z.; Markoulatos, P. (2008)The genetic properties of strain K/2002, isolated from fecal samples of a 7-month-old child who had received his first oral poliovirus vaccine (OPV) dose at the age of 3 months, are described. Preliminary sequencing ... -
Correlation of mutations and recombination with growth kinetics of poliovirus vaccine strains
Pliaka, V.; Kyriakopoulou, Z.; Tsakogiannis, D.; Ruether, I. G. A.; Gartzonika, C.; Levidiotou-Stefanou, S.; Krikelis, A.; Markoulatos, P. (2010)Attenuated strains of Sabin poliovirus vaccine replicate in the human gut and, in rare cases, may cause vaccine-associated paralytic poliomyelitis (VAPP). The genetic instability of Sabin strains constitutes one of the ... -
Determination of antigenic properties of vaccine derived poliovirus strains
Pliaka, V.; Achilleos, C.; Kyriakopoulou, Z.; Tsakogiannis, D.; Ruether, I. G. A.; Gartzonica, C.; Levidiotou-Stefanou, S.; Markoulatos, P. (2010)In this study, the immunity level of the southern Greek population in the 1-10-year, 11-20-year, 21-30-year and 31-40-year age groups with regard to Sabin vaccine strains and a collection of 11 recombinant and three ... -
Direct extraction and molecular characterization of enteroviruses genomes from human faecal samples
Bolanaki, E.; Kottaridi, C.; Dedepsidis, E.; Kyriakopoulou, Z.; Pliaka, V.; Pratti, A.; Levidiotou-Stefanou, S.; Markoulatos, P. (2008)Routine diagnosis of acute flaccid paralysis (AFP) is still based on classical virological procedures. Several enteroviruses serotypes are not easily isolated in cell cultures system used and routinely more than one passage ... -
Enteroviral infection in Greek AIDS patients
Papaventsis, D.; Markoulatos, P.; Mangafas, N.; Lazanas, M.; Levidiotou-Stefanou, S. (2004)Objective: Prolonged intestinal replication of polioviruses has not previously been studied in Greek AIDS patients. The objective of our study was to estimate the prevalence of enteroviral infections in this population. ... -
Environmental surveillance of circulation of enteroviruses-Public health effects
Papaventsis, D.; Siafakas, N.; Markoulatos, P.; Sopidou, V.; Economou, C.; Levidiotou-Stefanou, S. (2006)Objective: This study presents a new approach for the molecular "serotyping" of enteroviruses concentrated and isolated from urban sewage. Method: Samples were collected from the Nicosia Sewage Treatment Plant in Cyprus. ... -
Full-Genome Sequence Analysis of a Multirecombinant Echovirus 3 Strain Isolated from Sewage in Greece
Kyriakopoulou, Z.; Dedepsidis, E.; Pliaka, V.; Tsakogiannis, D.; Pratti, A.; Levidiotou-Stefanou, S.; Markoulatos, P. (2010)An echovirus 3 (Echo3) strain (strain LR31G7) was isolated from a sewage treatment plant in Greece in 2005. Full-genome molecular, phylogenetic, and SimPlot analyses were conducted in order to reveal the evolutionary ... -
Genome analysis of two type 6 echovirus (E6) strains recovered from sewage specimens in Greece in 2006
Kyriakopoulou, Z.; Pliaka, V.; Tsakogiannis, D.; Ruether, I. G. A.; Komiotis, D.; Gartzonika, C.; Levidiotou-Stefanou, S.; Markoulatos, P. (2012)Echovirus 6 (E6) is one of the main enteroviral serotypes that was isolated from cases of aseptic meningitis and encephalitis during the last years in Greece. Two E6 (LR51A5 and LR61G3) were isolated from the sewage treatment ... -
Growth kinetic analysis of bi-recombinant poliovirus vaccine strains
Pliaka, V.; Dedepsidis, E.; Kyriakopoulou, Z.; Papadi, G.; Tsakogiannis, D.; Pratti, A.; Levidiotou-Stefanou, S.; Markoulatos, P. (2010)Attenuated strains of Sabin poliovirus vaccine replicate in the human gut and in rare cases may cause vaccine-associated paralytic poliomyelitis (VAPP). Mutations at specific sites of the genome and recombination between ... -
Molecular and phylogenetic analysis of the HPV 16 E4 gene in cervical lesions from women in Greece
Tsakogiannis, D.; Ruether, I. G. A.; Kyriakopoulou, Z.; Pliaka, V.; Skordas, V.; Gartzonika, C.; Levidiotou-Stefanou, S.; Markoulatos, P. (2012)The HPV16 E1(a )E4 protein is thought to contribute to the release of newly formed viral particles from infected epithelia. In order to investigate amino acid mutations in the HPV16 E1(a )E4 protein, the complete E4 ORF ... -
Molecular characterization of wild-type polioviruses isolated in Greece during the 1996 outbreak in Albania
Kyriakopoulou, Z.; Kottaridi, C.; Dedepsidis, E.; Bolanaki, E.; Levidiotou-Stefanou, S.; Markoulatos, P. (2006)During the present study three type 1 poliovirus strains isolated in Greece during the 1996 poliomyelitis outbreak in Albania were retrospectively investigated and determination of their relationship with other epidemic ... -
Molecular epidemiology and evolutionary dynamics of Echovirus 3 serotype
Kyriakopoulou, Z.; Bletsa, M.; Tsakogiannis, D.; Dimitriou, T. G.; Amoutzias, G. D.; Gartzonika, C.; Levidiotou-Stefanou, S.; Markoulatos, P. (2015)Echovirus 3 (E3) serotype has been related with several neurologic diseases, although it constitutes one of the rarely isolated serotypes, with no report of epidemics in Europe. The aim of the present study was to provide ... -
Molecular identification and full genome analysis of an echovirus 7 strain isolated from the environment in Greece
Kyriakopoulou, Z.; Dedepsidis, E.; Pliaka, V.; Mastorakos, P.; Stamati, A.; Pratti, A.; Levidiotou-Stefanou, S.; Markoulatos, P. (2010)Two enteroviruses from river water and four from sewage treatment plant were isolated in Larissa, Greece, that all shared the same sequence. A full genome analysis was conducted in an attempt to reveal the evolutionary ... -
A new RT-PCR assay for the identification of the predominant recombination types in 2C and 3D genomic regions of vaccine-derived poliovirus strains
Pliaka, V.; Dedepsidis, E.; Kyriakopoulou, Z.; Mpirli, K.; Tsakogiannis, D.; Pratti, A.; Levidiotou-Stefanou, S.; Markoulatos, P. (2010)In the post-eradication era of wild polioviruses, the only remaining sources of poliovirus infection worldwide would be the vaccine-derived polioviruses (VDPVs). As the preponderance of countries certified to be polio-free ... -
Novel molecular techniques for the identification of the members of Brucellaceae family
Papaventsis, D.; Siafakas, N.; Markoulatos, P.; Sopidou, V.; Economou, C.; Levidiotou-Stefanou, S. (2006)An account is presented of an attempt to identify a multiresistant, gram-negative bacterium that was isolated from raw sewage in Cyprus. The API 2ONE system was initially used to identify the strain on the basis of its ... -
PCR assays for the identification of rare recombination types from VP1 to 3D genomic region of vaccine derived poliovirus strains
Dimitriou, T. G.; Pliaka, V.; Kyriakopoulou, Z.; Ruether, I. G. A.; Tsakogiannis, D.; Fountoucidou, P.; Gartzonika, C.; Levidiotou-Stefanou, S.; Markoulatos, P. (2014)Poliomyelitis has been effectively controlled by the use of inactivated poliovirus vaccine (IPV) or trivalent live attenuated oral poliovirus vaccine (OPV). Since 1964, the use of OPV in mass vaccinations has resulted in ... -
Retrospective characterization of a vaccine-derived poliovirus type 1 isolate from sewage in greece
Dedepsidis, E.; Kyriakopoulou, Z.; Pliaka, V.; Kottaridi, C.; Bolanaki, E.; Levidiotou-Stefanou, S.; Komiotis, D.; Markoulatos, P. (2007)Retrospective molecular and phenotypic characterization of a vaccine-derived poliovirus (VDPV) type 1 isolate (7/b/97) isolated from sewage in Athens, Greece, in 1997 is reported. VP1 sequencing of this isolate revealed ...