Πλοήγηση ανά Θέμα "RNA"

Low complexity regions in the proteins of prokaryotes perform important functional roles and are highly conserved

Ntountoumi C., Vlastaridis P., Mossialos D., Stathopoulos C., Iliopoulos I., Promponas V., Oliver S.G., Amoutzias G.D. (2019)

We provide the first high-throughput analysis of the properties and functional role of Low Complexity Regions (LCRs) in more than 1500 prokaryotic and phage proteomes. We observe that, contrary to a widespread belief based ...

Marine microbial community structure assessed from combined metagenomic analysis and ribosomal amplicon deep-sequencing

Genitsaris S., Monchy S., Denonfoux J., Ferreira S., Kormas K.A., Sime-Ngando T., Viscogliosi E., Christaki U. (2016)

The microbial taxonomic composition of the three domains of life in two coastal plankton samples was assessed by random total community metagenomic sequencing and PCR-based rDNA amplicon deep-sequencing in order to compare ...

Meta-analysis of gene expression data in adipose tissue reveals new obesity associated genes

Goutzelas Y., Kontou P., Mamuris Z., Bagos P., Sarafidou T. (2022)

High-throughput transcriptomic and proteomic data like microarray data are deposited in public databases such as Gene Expression Omnibus (GEO). Omics data integration and processing from different and independent studies ...

Microbial adaptation to high ammonia concentrations during anaerobic digestion of manure-based feedstock: biomethanation and 16S rRNA gene sequencing

Kalamaras S.D., Vasileiadis S., Karas P., Angelidaki I., Kotsopoulos T.A. (2020)

BACKGROUND: Microbial consortia resistant to high ammonia concentrations may facilitate biogas production from high ammonia-containing wastes and manures during anaerobic digestion. Microbial communities were gradually ...

Microbiological confinement of two adjacent water wells in Lake Karla Basin, Greece

Kormas K.A., Meziti A., Papadimitriou T. (2015)

We analyzed the bacterial and archaeal community structure of two adjacent irrigation well waters of the Lake Karla Basin, Central Greece, in order to elucidate their connectivity or confinement by using 454 tag pyrosequencing ...

Microbiological status based on 454-pyrosequencing and volatilome analysis of gilthead seabream (Sparus aurata) fed on diets with hydrolyzed feather meal and poultry by-product meal as fishmeal replacers

Parlapani F.F., Boziaris I.S., Meziti A., Michailidou S., Haroutounian S.A., Argiriou A., Karapanagiotidis I.T. (2019)

The effect of two experimental diets with different animal protein sources on sensory, microbiological and chemical attributes of Sparus aurata flesh stored at chilling temperatures were explored through sensory evaluation, ...

microCLIP super learning framework uncovers functional transcriptome-wide miRNA interactions

Paraskevopoulou M.D., Karagkouni D., Vlachos I.S., Tastsoglou S., Hatzigeorgiou A.G. (2018)

Argonaute crosslinking and immunoprecipitation (CLIP) experiments are the most widely used high-throughput methodologies for miRNA targetome characterization. The analysis of Photoactivatable Ribonucleoside-Enhanced (PAR) ...

microTSS: Accurate microRNA transcription start site identification reveals a significant number of divergent pri-miRNAs

Georgakilas, G.; Vlachos, I. S.; Paraskevopoulou, M. D.; Yang, P.; Zhang, Y.; Economides, A. N.; Hatzigeorgiou, A. G. (2014)

A large fraction of microRNAs (miRNAs) are derived from intergenic non-coding loci and the identification of their promoters remains 'elusive'. Here, we present microTSS, a machine-learning algorithm that provides highly ...

MirPub v2: Towards ranking and refining miRNA publication search results

Kanellos I., Vlachokyriakou V., Vergoulis T., Georgakilas G., Vassiliou Y., Hatzigeorgiou A.K., Dalamagas T. (2015)

In recent years, many articles studying microRNA (miRNA) molecules and their connection to diseases have been published. However, the wide range of literature in life sciences, raises a barrier in extracting useful information ...

Mitochondrial tRNA mutations: Clinical and functional perturbations

Zifa, E.; Giannouli, S.; Theotokis, P.; Stamatis, C.; Mamuris, Z.; Stathopoulos, C. (2007)

During the last decade, there has been a progressive accumulation of reports that connect the identification of specific mitochondrial tRNA gene mutations to severe disorders in human. As a result, mitochondrial tRNA genes ...

Mobility and potential bioavailability of antimony in contaminated soils: Short-term impact on microbial community and soil biochemical functioning

Diquattro S., Garau G., Mangia N.P., Drigo B., Lombi E., Vasileiadis S., Castaldi P. (2020)

Antimony (Sb) and its compounds are emerging priority pollutants which pose a serious threat to the environment. The aim of this study was to evaluate the short-term fate of antimonate added to different soils (S1 and S2) ...

Monitoring of spoilage and determination of microbial communities based on 16S rRNA gene sequence analysis of whole sea bream stored at various temperatures

Parlapani F.F., Boziaris I.S. (2016)

Exploration of initial and spoilage microbiota grown on plates of whole sea bream stored aerobically at 0 (ice), 5 and 15 °C, was conducted by 16S rRNA gene sequence analysis. The course of spoilage was recorded by monitoring ...

A multifunctional RNA recognition motif in poly(A)-specific ribonuclease with cap and poly(A) binding properties

Nilsson, P.; Henriksson, N.; Niedzwiecka, A.; Balatsos, N. A. A.; Kokkoris, K.; Eriksson, J.; Virtanen, A. (2007)

Poly(A)-specific ribonuclease (PARN) is an oligomeric, processive and cap-interacting 3′ exoribonuclease that efficiently degrades mRNA poly(A) tails. Here we show that the RNA recognition motif (RRM) of PARN harbors both ...

Muscle xenografts reproduce key molecular features of facioscapulohumeral muscular dystrophy

Mueller A.L., O'Neill A., Jones T.I., Llach A., Rojas L.A., Sakellariou P., Stadler G., Wright W.E., Eyerman D., Jones P.L., Bloch R.J. (2019)

Aberrant expression of DUX4, a gene unique to humans and primates, causes Facioscapulohumeral Muscular Dystrophy-1 (FSHD), yet the pathogenic mechanism is unknown. As transgenic overexpression models have largely failed ...

Nanopore long-read RNA-seq and absolute quantification delineate transcription dynamics in early embryo development of an insect pest

Bayega A., Oikonomopoulos S., Gregoriou M.-E., Tsoumani K.T., Giakountis A., Wang Y.C., Mathiopoulos K.D., Ragoussis J. (2021)

The olive fruit fly, Bactrocera oleae, is the most important pest for the olive fruit but lacks adequate transcriptomic characterization that could aid in molecular control approaches. We apply nanopore long-read RNA-seq ...

Network analysis in aged C. elegans reveals candidate regulatory genes of ageing

Aktypi F., Papaevgeniou N., Voutetakis K., Chatziioannou A., Grune T., Chondrogianni N. (2021)

Ageing is a biological process guided by genetic and environmental factors that ultimately lead to adverse outcomes for organismal lifespan and healthspan. Determination of molecular pathways that are affected with age and ...

A novel deep intronic SERPING1 variant as a cause of hereditary angioedema due to C1-inhibitor deficiency

Vatsiou S., Zamanakou M., Loules G., Psarros F., Parsopoulou F., Csuka D., Valerieva A., Staevska M., Porebski G., Obtulowicz K., Magerl M., Maurer M., Speletas M., Farkas H., Germenis A.E. (2020)

Background: In about 5% of patients with hereditary angioedema due to C1-inhibitor deficiency (C1-INH-HAE) no mutation in the SERPING1 gene is detected. Methods: C1-INH-HAE cases with no mutation in the coding region of ...

Parkinson's disease and pesticides: Are microRNAs the missing link?

Aloizou A.-M., Siokas V., Sapouni E.-M., Sita N., Liampas I., Brotis A.G., Rakitskii V.N., Burykina T.I., Aschner M., Bogdanos D.P., Tsatsakis A., Hadjigeorgiou G.M., Dardiotis E. (2020)

Parkinson's disease (PD) is a common neurodegenerative disorder that leads to significant morbidity and decline in the quality of life. It develops due to loss of dopaminergic neurons in the substantia nigra pars compacta, ...

PCR assays for the identification of rare recombination types from VP1 to 3D genomic region of vaccine derived poliovirus strains

Dimitriou, T. G.; Pliaka, V.; Kyriakopoulou, Z.; Ruether, I. G. A.; Tsakogiannis, D.; Fountoucidou, P.; Gartzonika, C.; Levidiotou-Stefanou, S.; Markoulatos, P. (2014)

Poliomyelitis has been effectively controlled by the use of inactivated poliovirus vaccine (IPV) or trivalent live attenuated oral poliovirus vaccine (OPV). Since 1964, the use of OPV in mass vaccinations has resulted in ...

Positioning Europe for the EPITRANSCRIPTOMICS challenge

Jantsch M.F., Quattrone A., O'Connell M., Helm M., Frye M., Macias-Gonzales M., Ohman M., Ameres S., Willems L., Fuks F., Oulas A., Vanacova S., Nielsen H., Bousquet-Antonelli C., Motorin Y., Roignant J.-Y., Balatsos N., Dinnyes A., Baranov P., Kelly V., Lamm A., Rechavi G., Pelizzola M., Liepins J., Holodnuka Kholodnyuk I., Zammit V., Ayers D., Drablos F., Dahl J.A., Bujnicki J., Jeronimo C., Almeida R., Neagu M., Costache M., Bankovic J., Banovic B., Kyselovic J., Valor L.M., Selbert S., Pir P., Demircan T., Cowling V., Schäfer M., Rossmanith W., Lafontaine D., David A., Carre C., Lyko F., Schaffrath R., Schwartz S., Verdel A., Klungland A., Purta E., Timotijevic G., Cardona F., Davalos A., Ballana E., O'Carroll D., Ule J., Fray R. (2018)

The genetic alphabet consists of the four letters: C, A, G, and T in DNA and C,A,G, and U in RNA. Triplets of these four letters jointly encode 20 different amino acids out of which proteins of all organisms are built. ...