Parcourir par sujet "Western blotting"
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An Activating Janus kinase-3 mutation is associated with cytotoxic T lymphocyte antigen-4-dependent Immune dysregulation syndrome
(2017)Heterozygous mutations in the cytotoxic T lymphocyte antigen-4 (CTLA-4) are associated with lymphadenopathy, autoimmunity, immune dysregulation, and hypogammaglobulinemia in about 70% of the carriers. So far, the incomplete ... -
Activation of FADD-Dependent neuronal death pathways as a predictor of pathogenicity for LRRK2 mutations
(2016)Background Despite the plethora of sequence variants in LRRK2, only a few clearly segregate with PD. Even within this group of pathogenic mutations, the phenotypic profile can differ widely. Objective We examined multiple ... -
Activation of general control nonderepressible 2 kinase protects human glomerular endothelial cells from harmful high-glucose-induced molecular pathways
(2016)Purpose: Considering the referred beneficial effects of protein restriction on diabetic nephropathy (DN) and the role of renal endothelium in its pathogenesis, we evaluated the effect of general control nonderepressible 2 ... -
Activation of general control nonderepressible-2 kinase ameliorates glucotoxicity in human peritoneal mesothelial cells, preserves their integrity, and prevents mesothelial to mesenchymal transition
(2019)Along with infections, ultrafiltration failure due to the toxicity of glucose-containing peritoneal dialysis (PD) solutions is the Achilles’ heel of PD method. Triggered by the protective effect of general control ... -
Alanyl-glutamine restores tight junction organization after disruption by a conventional peritoneal dialysis fluid
(2020)Understanding and targeting the molecular basis of peritoneal solute and protein transport is essential to improve peritoneal dialysis (PD) efficacy and patient outcome. Supplementation of PD fluids (PDF) with alanyl-glutamine ... -
Aldehyde dehydrogenase 1B1: Molecular cloning and characterization of a novel mitochondrial acetaldehyde-metabolizing enzyme
(2010)Ethanol-induced damage is largely attributed to its toxic metabolite, acetaldehyde. Clearance of acetaldehyde is achieved by its oxidation, primarily catalyzed by the mitochondrial class II aldehyde dehydrogenase (ALDH2). ... -
Angiogenin is upregulated during the alloreactive immune response and has no effect on the T-cell expansion phase, whereas it affects the contraction phase by inhibiting CD4+ T-cell apoptosis
(2016)Under growth conditions, angiogenin is translocated into the nucleus, where it enhances ribosomal RNA transcription, facilitating increased protein synthesis and cellular proliferation. During stress conditions, angiogenin ... -
Antioxidant effects of an olive oil total polyphenolic fraction from a Greek Olea europaea variety in different cell cultures
(2018)Background: Numerous studies have been carried out concerning the advantageous health effects, especially the antioxidant effects, of olive oil's (OO) individual biophenolic compounds, but none until now for its total ... -
A battery of translational biomarkers for the assessment of the in vitro and in vivo antioxidant action of plant polyphenolic compounds: The biomarker issue
(2019)Over the last decades, the scientific findings stressing the beneficial health implications of plant-derived compounds (i.e., plant extracts rich in polyphenols) have been increased significantly. Assessing the properties ... -
Biofunctional Feed Supplemented with By-products of Olive Oil Production Improves Tissue Antioxidant Profile of Lambs
(2020)Background/Aim: Olive mill wastewater (OMW) is a byproduct of olive oil production. The aim of the study was to estimate the redox profile of lambs' vital organs after consumption of an OMW-supplemented feed. Materials and ... -
Brattleboro rats have impaired apical membrane water permeability regulation in the outer medullary collecting duct principal cells
(2016)Vasopressin (AVP) regulates the body salt-water balance. Brattleboro rats carry an AVP gene mutation resulting in a recessive form of central diabetes insipidus, being ideal for AVP deficiency studies. Herein, we studied ... -
CB1R-dependent activation of Fyn tyrosine kinase and protein kinase C Delta, PKCδ, in lipid rafts
(2008)Cannabinoid 1 receptors (CB1Rs) are heptahelical transmembrane receptors which may exert their effects through the activation of the extracellular signal-regulated kinases (ERKs). We have previously shown that stably ... -
Comparative proteomic analysis of hypertrophic chondrocytes in osteoarthritis
(2015)Background: Osteoarthritis (OA) is a multi-factorial disease leading progressively to loss of articular cartilage and subsequently to loss of joint function. While hypertrophy of chondrocytes is a physiological process ... -
Crystalline silica activates the T-cell and the B-cell antigen receptor complexes and induces T-cell and B-cell proliferation
(2019)Silicosis is an occupational fibrotic lung disease, which is associated with an increased incidence of autoimmune diseases. The effect of crystalline silica on the immune system is thought to be mediated by the antigen ... -
Dietary prenylated flavonoid xanthohumol alleviates oxidative damage and accelerates diabetic wound healing via Nrf2 activation
(2022)Diabetic skin ulcer is one of the most common complications in patients suffering diabetes mellitus. Xanthohumol (XN), a hop-derived prenylated dietary flavonoid, has multiple health beneficial bioactivities. In the present ... -
Differential expression of leptin and leptin's receptor isoform (Ob-Rb) mRNA between advanced and minimally affected osteoarthritic cartilage; effect on cartilage metabolism
(2007)Objective: To investigate leptin's effect on cartilage metabolism and the pathophysiology of osteoarthritis (OA). Methods: Messenger RNA (mRNA) expression and protein levels of leptin and leptin's receptor isoforms were ... -
Dysregulation of caveolin-1 phosphorylation and nuclear translocation is associated with senescence onset
(2021)We recently reported that the inability of osteoarthritic (OA) chondrocytes to repair oxidative stress (OS) induced DNA damage is linked to Cav-1 overexpression/improper localization. We speculated that the senescent status ... -
Energy handling in renal tubular epithelial cells of the hamster, a native hibernator, under warm anoxia or reoxygenation
(2018)Ischemia-reperfusion (I-R) injury causes several diseases, including acute kidney injury. Hibernating mammals survive periods of torpor with a marked drop in tissue perfusion, interspersed with periods of arousal, and ... -
Enhanced Ca2+ Entry Sustains the Activation of Akt in Glucose Deprived SH-SY5Y Cells
(2022)The two crucial cellular insults that take place during cerebral ischemia are the loss of oxygen and loss of glucose, which can both activate a cascade of events leading to neuronal death. In addition, the toxic overactivation ... -
Evidence for disulfide bonds in SR Protein Kinase 1 (SRPK1) that are required for activity and nuclear localization
(2017)Serine/arginine protein kinases (SRPKs) phosphorylate Arg/Ser dipeptide-containing proteins that play crucial roles in a broad spectrum of basic cellular processes. The existence of a large internal spacer sequence that ...