Parcourir par auteur "Dedepsidis, E."
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Complete genomic characterization of an intertypic Sabin 3/Sabin 2 capsid recombinant
Dedepsidis, E.; Pliaka, V.; Kyriakopoulou, Z.; Brakoulias, C.; Levidiotou-Stefanou, S.; Pratti, A.; Mamuris, Z.; Markoulatos, P. (2008)The genetic properties of strain K/2002, isolated from fecal samples of a 7-month-old child who had received his first oral poliovirus vaccine (OPV) dose at the age of 3 months, are described. Preliminary sequencing ... -
Complete nucleotide sequence analysis of the VP1 genomic region of Echoviruses 6 isolated from sewage in Greece revealed 98% similarity with Echoviruses 6 that were characterized from an aseptic meningitis outbreak 1 year later
Kyriakopoulou, Z.; Dedepsidis, E.; Pliaka, V.; Tsakogiannis, D.; Ruether, I. G. A.; Krikelis, A.; Markoulatos, P. (2011)The molecular characterization of two enterovirus strains (LR51A5 and LR61G3) isolated from the sewage treatment plant unit in Larissa, Greece, in May and June 2006 and the investigation of their relationship with enteroviruses ... -
Correlation between recombination junctions and RNA secondary structure elements in poliovirus Sabin strains
Dedepsidis, E.; Kyriakopoulou, Z.; Pliaka, V.; Markoulatos, P. (2010)In order to test the hypothesis that RNA structural elements promote the distribution of certain types of recombination junctions in each one of the 2C and 3D poliovirus genomic regions (Sabin 3/Sabin 2 or Sabin 1 in 2C ... -
Detection of unusual mutation within the VP1 region of different re-isolates of poliovirus Sabin vaccine
Dedepsidis, E.; Karakasiliotis, I.; Paximadi, E.; Kyriakopoulou, Z.; Komiotis, D.; Markoulatos, P. (2006)In the present study, a genomic analysis of full VP1 sequence region of 15 clinical re-isolates (14 healthy vaccinees and one bone marrow tumor patient) was conducted, aiming to the identification of mutations and to the ... -
Direct extraction and molecular characterization of enteroviruses genomes from human faecal samples
Bolanaki, E.; Kottaridi, C.; Dedepsidis, E.; Kyriakopoulou, Z.; Pliaka, V.; Pratti, A.; Levidiotou-Stefanou, S.; Markoulatos, P. (2008)Routine diagnosis of acute flaccid paralysis (AFP) is still based on classical virological procedures. Several enteroviruses serotypes are not easily isolated in cell cultures system used and routinely more than one passage ... -
Full-Genome Sequence Analysis of a Multirecombinant Echovirus 3 Strain Isolated from Sewage in Greece
Kyriakopoulou, Z.; Dedepsidis, E.; Pliaka, V.; Tsakogiannis, D.; Pratti, A.; Levidiotou-Stefanou, S.; Markoulatos, P. (2010)An echovirus 3 (Echo3) strain (strain LR31G7) was isolated from a sewage treatment plant in Greece in 2005. Full-genome molecular, phylogenetic, and SimPlot analyses were conducted in order to reveal the evolutionary ... -
Growth kinetic analysis of bi-recombinant poliovirus vaccine strains
Pliaka, V.; Dedepsidis, E.; Kyriakopoulou, Z.; Papadi, G.; Tsakogiannis, D.; Pratti, A.; Levidiotou-Stefanou, S.; Markoulatos, P. (2010)Attenuated strains of Sabin poliovirus vaccine replicate in the human gut and in rare cases may cause vaccine-associated paralytic poliomyelitis (VAPP). Mutations at specific sites of the genome and recombination between ... -
Molecular characterization of wild-type polioviruses isolated in Greece during the 1996 outbreak in Albania
Kyriakopoulou, Z.; Kottaridi, C.; Dedepsidis, E.; Bolanaki, E.; Levidiotou-Stefanou, S.; Markoulatos, P. (2006)During the present study three type 1 poliovirus strains isolated in Greece during the 1996 poliomyelitis outbreak in Albania were retrospectively investigated and determination of their relationship with other epidemic ... -
Molecular identification and full genome analysis of an echovirus 7 strain isolated from the environment in Greece
Kyriakopoulou, Z.; Dedepsidis, E.; Pliaka, V.; Mastorakos, P.; Stamati, A.; Pratti, A.; Levidiotou-Stefanou, S.; Markoulatos, P. (2010)Two enteroviruses from river water and four from sewage treatment plant were isolated in Larissa, Greece, that all shared the same sequence. A full genome analysis was conducted in an attempt to reveal the evolutionary ... -
A new RT-PCR assay for the identification of the predominant recombination types in 2C and 3D genomic regions of vaccine-derived poliovirus strains
Pliaka, V.; Dedepsidis, E.; Kyriakopoulou, Z.; Mpirli, K.; Tsakogiannis, D.; Pratti, A.; Levidiotou-Stefanou, S.; Markoulatos, P. (2010)In the post-eradication era of wild polioviruses, the only remaining sources of poliovirus infection worldwide would be the vaccine-derived polioviruses (VDPVs). As the preponderance of countries certified to be polio-free ... -
Possible recombination and gene adaptation exchanges among clinical echovirus strains: crossing the temporal and topological barriers
Kottaridi, C.; Bolanaki, E.; Kyriakopoulou, Z.; Dedepsidis, E.; Pratti, A.; Markoulatos, P. (2007)Six echovirus strains belonging to serotypes echovirus 6, 13, and 30 were investigated in the present work by sequencing of the whole 2C gene and about 560 nt of the 5' part of 3-dimensional genomic region. Four of the 6 ... -
Retrospective characterization of a vaccine-derived poliovirus type 1 isolate from sewage in greece
Dedepsidis, E.; Kyriakopoulou, Z.; Pliaka, V.; Kottaridi, C.; Bolanaki, E.; Levidiotou-Stefanou, S.; Komiotis, D.; Markoulatos, P. (2007)Retrospective molecular and phenotypic characterization of a vaccine-derived poliovirus (VDPV) type 1 isolate (7/b/97) isolated from sewage in Athens, Greece, in 1997 is reported. VP1 sequencing of this isolate revealed ... -
Use of mutational pattern in 5 '-NCR and VP1 regions of polioviruses for molecular diagnosis
Pliaka, V.; Dedepsidis, E.; Kyriakopoulou, Z.; Papadopoulou, I.; Levidiotou, S.; Markoulatos, P. (2007)Polioviruses are members of the enterovirus genus, belonging to the Picornaviridae family. They are the causative agents of poliomyelitis, a paralytic and sometimes fatal disease in humans. The number of poliomyelitis cases ...