Parcourir par auteur "Bolanaki, E."
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Amplification of Echoviruses genomic regions by different RT-PCR protocols - a comparative study
Kottaridi, C.; Bolanaki, E.; Markoulatos, P. (2004)In the present report, the results of a comparative study in the detection of all Echoviruses reference strains as well as of 38 clinical isolates are presented. Using RT-PCR with already published primer pairs (UG(52)-UC53, ... -
A comparative amplification of five different genomic regions on Coxsackie A and B viruses. Implications in clinical diagnostics
Bolanaki, E.; Kottaridi, C.; Markoulatos, P.; Margaritis, L.; Katsorchis, T. (2005)Modern molecular approaches in Human Enterovirus detection rely on the designing of generic and often degenerate primers in order to amplify specific sequences within the enterovirus genome. In the present study a comparative ... -
Direct extraction and molecular characterization of enteroviruses genomes from human faecal samples
Bolanaki, E.; Kottaridi, C.; Dedepsidis, E.; Kyriakopoulou, Z.; Pliaka, V.; Pratti, A.; Levidiotou-Stefanou, S.; Markoulatos, P. (2008)Routine diagnosis of acute flaccid paralysis (AFP) is still based on classical virological procedures. Several enteroviruses serotypes are not easily isolated in cell cultures system used and routinely more than one passage ... -
Evaluation of seroneutralization and molecular diagnostic methods for echovirus identification
Kottaridi, C.; Bolanaki, E.; Siafakas, N.; Markoulatos, P. (2005)In this study we compared the identification results of 41 echovirus clinical isolates using RIVM pools (National Institute for Public Health and the Environment RIVM, Bilthoven, The Netherlands) and reverse transcriptio ... -
Evolution of 2B and 2C genomic parts of species B Coxsackie viruses. Phylogenetic study and comparison with other regions
Bolanaki, E.; Kottaridi, C.; Markoulatos, P.; Margaritis, L.; Katsorchis, T. (2006)Modern molecular approaches on the genome of enteroviruses' circulating strains have established new data about the mechanism and significance of its evolution. In the present study, 46 enteroviruses isolates, belonging ... -
Molecular characterization of wild-type polioviruses isolated in Greece during the 1996 outbreak in Albania
Kyriakopoulou, Z.; Kottaridi, C.; Dedepsidis, E.; Bolanaki, E.; Levidiotou-Stefanou, S.; Markoulatos, P. (2006)During the present study three type 1 poliovirus strains isolated in Greece during the 1996 poliomyelitis outbreak in Albania were retrospectively investigated and determination of their relationship with other epidemic ... -
Molecular phylogeny of VP1, 2A, and 2B genes of echovirus isolates: epidemiological linkage and observations on genetic variation
Kottaridi, C.; Bolanaki, E.; Mamuris, Z.; Stathopoulos, C.; Markoulatos, P. (2006)Phylogenetic relationships between 37 echovirus clinical isolates, most of them originating from an aseptic meningitis outbreak during 2001 in Greece, were investigated by RT-PCR and sequencing. The generic primers 292 and ... -
Nucleotide analysis and phylogenetic study of the homology boundaries of coxsackie A and B viruses
Bolanaki, E.; Kottaridi, C.; Markoulatos, P.; Margaritis, L.; Katsorchis, T. (2005)Modern molecular methods use VP1 coding region as a target for RT-PCR assays followed by sequencing, in order to identify new untyped enteroviruses' strains. In the present study, two different genomic portions of VP1 and ... -
Partial 3D gene sequences of Coxsackie viruses reveal interspecies exchanges
Bolanaki, E.; Kottaridi, C.; Markoulatos, P.; Kyriakopoulou, Z.; Margaritis, L.; Katsorchis, T. (2007)The 3D region of 46 clinical Coxsackievirus strains, primarily belonging to the human enterovirus B species (HEV-B), were analyzed using nucleotide distance matrices and phylogeny software. The conclusions from previously ... -
Possible recombination and gene adaptation exchanges among clinical echovirus strains: crossing the temporal and topological barriers
Kottaridi, C.; Bolanaki, E.; Kyriakopoulou, Z.; Dedepsidis, E.; Pratti, A.; Markoulatos, P. (2007)Six echovirus strains belonging to serotypes echovirus 6, 13, and 30 were investigated in the present work by sequencing of the whole 2C gene and about 560 nt of the 5' part of 3-dimensional genomic region. Four of the 6 ... -
Retrospective characterization of a vaccine-derived poliovirus type 1 isolate from sewage in greece
Dedepsidis, E.; Kyriakopoulou, Z.; Pliaka, V.; Kottaridi, C.; Bolanaki, E.; Levidiotou-Stefanou, S.; Komiotis, D.; Markoulatos, P. (2007)Retrospective molecular and phenotypic characterization of a vaccine-derived poliovirus (VDPV) type 1 isolate (7/b/97) isolated from sewage in Athens, Greece, in 1997 is reported. VP1 sequencing of this isolate revealed ... -
Vaccine derived bi- and multi-recombinant Sabin strains
Paximadi, E.; Karakasiliotis, I.; Bolanaki, E.; Krikelis, A.; Markoulatos, P. (2007)A retrospective analysis of five Sabin intertypic recombinant strains, isolated from human feacal specimens during the time period 1978-1985 in Greece, was performed by RT-PCR, Restriction Fragment Length Polymorphism ...