Listar por tema "polyacrylamide gel electrophoresis"
Mostrando ítems 1-19 de 19
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An Activating Janus kinase-3 mutation is associated with cytotoxic T lymphocyte antigen-4-dependent Immune dysregulation syndrome
(2017)Heterozygous mutations in the cytotoxic T lymphocyte antigen-4 (CTLA-4) are associated with lymphadenopathy, autoimmunity, immune dysregulation, and hypogammaglobulinemia in about 70% of the carriers. So far, the incomplete ... -
Application of Bayesian modeling for diagnostic assays of Mycobacterium avium subsp. paratuberculosis in sheep and goats flocks
(2022)Background: This study aimed to screen the sera of goats and sheep from flocks suspected of Mycobacterium avium subsp. paratuberculosis (MAP) infection by a newly standardized Mce-truncated ELISA (Mt-ELISA) kit for the ... -
Applied proteomics in companion animal medicine
(2016)Background: Proteomics in companion animal medicine has been used chiefly, in order to identify proteins, which may be used as biomarkers for early diagnosis of a variety of pathological conditions, as well as to elucidate ... -
Biochemical and in silico identification of the active site and the catalytic mechanism of the circadian deadenylase HESPERIN
(2022)The 24-h molecular clock is based on the stability of rhythmically expressed transcripts. The shortening of the poly(A) tail of mRNAs is often the first and rate-limiting step that determines the lifespan of a mRNA and is ... -
Chondrocyte protein co-synthesis network analysis links ECM mechanosensing to metabolic adaptation in osteoarthritis
(2021)Background: Knee osteoarthritis (OA) is one of the most common structural OA disorders globally. Incomplete understanding of the fundamental biological aspects of osteoarthritis underlies the current lack of effective ... -
Dietary prenylated flavonoid xanthohumol alleviates oxidative damage and accelerates diabetic wound healing via Nrf2 activation
(2022)Diabetic skin ulcer is one of the most common complications in patients suffering diabetes mellitus. Xanthohumol (XN), a hop-derived prenylated dietary flavonoid, has multiple health beneficial bioactivities. In the present ... -
Effect of storage time on the urine protein: creatinine ratio in alkaline ovine urine
(2021)The urine protein:creatinine (UPC) ratio is considered the reference method to assess proteinuria. Its diagnostic value in ovine medicine needs further elucidation. In population monitoring and/or for research purposes, ... -
Evidence for disulfide bonds in SR Protein Kinase 1 (SRPK1) that are required for activity and nuclear localization
(2017)Serine/arginine protein kinases (SRPKs) phosphorylate Arg/Ser dipeptide-containing proteins that play crucial roles in a broad spectrum of basic cellular processes. The existence of a large internal spacer sequence that ... -
Identification of MAPK phosphorylation sites and their role in the localization and activity of hypoxia-inducible factor-1α
(2006)Hypoxia-inducible factor 1 (HIF-1) controls the expression of most genes induced by hypoxic conditions. Regulation of expression and activity of its inducible subunit, HIF-1α, involves several post-translational modifications. ... -
Low-Grade Systemic Inflammation Interferes with Anabolic and Catabolic Characteristics of the Aged Human Skeletal Muscle
(2021)Aging is associated with the development of chronic low-grade systemic inflammation (LGSI) characterized by increased circulating levels of proinflammatory cytokines and acute phase proteins such as C-reactive protein ... -
Magnetic nanoparticles in medical diagnostic applications: Synthesis, characterization and proteins conjugation
(2016)Background: Magnetic nanoparticles (NPs) used in biomedical applications should be discrete with small particle sizes, narrow size distribution and superparamagnetic. NPs can be tailored to target, through chemical bonds, ... -
Mechanisms of action of differentiation inducers: Detection of inducer binding protein(s) in murine erythroleukemia cells
(2005)We have shown previously that murine erythroleukemia (MEL) and human neuroectodermal RD/TE-671 cells are induced to differentiate by ureido derivatives of pyridine (UDPs) and may contain inducer binding protein(s). In the ... -
Novel HIF-2α interaction with Reptin52 impairs HIF-2 transcriptional activity and EPO secretion
(2021)Hypoxia-inducible factor 2 (HIF-2), is essential for cellular response to hypoxia and holds an important role in erythropoiesis, angiogenesis, tissue invasion and metastasis, thus, constituting an important therapeutic ... -
Olive oil with high polyphenolic content induces both beneficial and harmful alterations on rat redox status depending on the tissue
(2020)Olive oil (OO) possesses a predominant role in the diet of Mediterranean countries. According to a health claim approved by the European Food Safety Authority, OO protects against oxidative stress‑induced lipid peroxidation ... -
Oxidation of human serum albumin exhibits inter-individual variability after an ultra-marathon mountain race
(2017)The aim of this study was to examine the oxidation of human serum albumin (HSA) caused by oxidative stress following exhaustive and demanding exercise, such as an ultra-marathon race. For this purpose, blood samples from ... -
Purification of a candidate gonadotrophin surge attenuating factor from human follicular fluid
(1999)Gonadotrophin surge attenuating factor (GnSAF) is a new non-steroidal ovarian substance, different from inhibin, which attenuates the pre-ovulatory luteinizing hormone (LH) surge in superovulated women. Human follicular ... -
Second-Generation Escherichia coli SuptoxR Strains for High-Level Recombinant Membrane Protein Production
(2022)Escherichia coli is one of the most widely utilized hosts for recombinant protein production, including that of membrane proteins (MPs). We have recently engineered a specialized E. coli strain for enhanced recombinant MP ... -
Unexpected similarities between C9ORF72 and sporadic forms of ALS/FTD suggest a common disease mechanism
(2018)Amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) represent two ends of a disease spectrum with shared clinical, genetic and pathological features. These include near ubiquitous pathological inclusions ... -
Use of proteomics in the study of mastitis in ewes
(2019)The objective of this review is to describe the usage and applicability of proteomics technologies in the study of mastitis in ewes. In ewes, proteomics technologies have been employed for furthering knowledge in mastitis ...