Browsing by Author "Stathopoulos, C."
Now showing items 1-20 of 38
-
A 40.7 kDa Rpp30/Rpp1 homologue is a protein subunit of Dictyostelium discoideum RNase P holoenzyme
Vourekas, A.; Kalavrizioti, D.; Zarkadis, I. K.; Spyroulias, G. A.; Stathopoulos, C.; Drainas, D. (2007)RNase P is an essential and ubiquitous endonuclease that mediates the maturation of the 5' ends of all precursor tRNA molecules. The holoenzyme from Dictyostelium discoideum possesses RNA and protein subunits essential for ... -
Asymmetric behavior of archaeal prolyl-tRNA synthetase
Ambrogelly, A.; Kamtekar, S.; Stathopoulos, C.; Kennedy, D.; Söll, D. (2005)Archaeal prolyl-tRNA synthetases differ from their bacterial counterparts: they contain an additional domain (about 70 amino acids) appended to the carboxy-terminus and lack an editing domain inserted into the class II ... -
Competitive Inhibition of Human Poly(A)-Specific Ribonuclease (PARN) by Synthetic Fluoro-Pyranosyl Nucleosides
Balatsos, N. A. A.; Vlachakis, D.; Maragozidis, P.; Manta, S.; Anastasakis, D.; Kyritsis, A.; Vlassi, M.; Komiotis, D.; Stathopoulos, C. (2009)Poly(A)-specific ribonuclease (PARN) is a cap-interacting deadenylase that mediates, together with other exonucleases, the eukaryotic mRNA turnover and thus is actively involved in the regulation of gene expression. ... -
Composition of the ribonucleoprotein complex of ribonuclease P from Dictyostelium discoideurn
Vourekas, A.; Kalavrizioti, D.; Theodorakopoulou, O.; Stamatopoulou, V.; Toumpeki, C.; Stathopoulos, C.; Drainas, D. (2006) -
Cytogenetic effects of grape extracts (Vitis vinifera) and polyphenols on mitomycin C-induced sister chromatid exchanges (SCEs) in human blood lymphocytes
Stagos, D.; Spanou, C.; Margariti, M.; Stathopoulos, C.; Mamuris, Z.; Kazantzoglou, G.; Magiatis, P.; Kouretas, D. (2007)In the present study, the effects of extracts and polyphenol-rich fractions as well as monomer polyphenols identified in them, from both red and white grapes, on mitomycin C (MMC) induced sister chromatid exchanges (SCEs) ... -
Detection of a phylogenetically distinct IMP-type metallo-beta-lactamase, IMP-35, in a CC235 Pseudomonas aeruginosa from the Dutch-German border region (Euregio)
Pournaras, S.; Kock, R.; Mossialos, D.; Mellmann, A.; Sakellaris, V.; Stathopoulos, C.; Friedrich, A. W.; Tsakris, A. (2013)To characterize a highly divergent IMP-type metallo--lactamase (MBL) variant detected in a multidrug-resistant Pseudomonas aeruginosa clinical isolate. P. aeruginosa isolate 1876 was recovered from an anal swab of an ... -
Detection of mutations in the FemXAB protein family in oxacillin-susceptible mecA-positive Staphylococcus aureus clinical isolates
Giannouli, S.; Labrou, M.; Kyritsis, A.; Ikonomidis, A.; Pournaras, S.; Stathopoulos, C.; Tsakris, A. (2010)Methicillin-resistant Staphylococcus aureus (MRSA) strains that express the mecA gene but are oxacillin susceptible (OS-MRSA; oxacillin MIC < 2 mg/L) are increasingly reported. To gain molecular and functional insights on ... -
Differences in biofilm formation and virulence factors between clinical and fecal enterococcal isolates of human and animal origin
Tsikrikonis, G.; Maniatis, A. N.; Labrou, M.; Ntokou, E.; Michail, G.; Daponte, A.; Stathopoulos, C.; Tsakris, A.; Pournaras, S. (2012)The present study investigated the possible correlation between carriage of the virulence genes esp and fsrb, production of hemolysin and gelatinase and biofilm formation in human vs. animal enterococcal isolates. A ... -
Effect of natural nucleotides and their synthetic analogues on mRNA turnover by human poly(A)specific ribonuclease (PARN)
Balatsos, N.; Anastasakis, D.; Maragozidis, P.; Manta, S.; Komiotis, D.; Stathopoulos, C. (2008) -
EFFECT OF TRANSOSSEOUS APPLICATION OF LOW-INTENSITY ULTRASOUND AT THE TENDON GRAFT-BONE INTERFACE HEALING: GENE EXPRESSION AND HISTOLOGICAL ANALYSIS IN RABBITS
Papatheodorou, L. K.; Malizos, K. N.; Poultsides, L. A.; Hantes, M. E.; Grafanaki, K.; Giannouli, S.; Ioannou, M. G.; Koukoulis, G. K.; Protopappas, V. C.; Fotiadis, D. I.; Stathopoulos, C. (2009)The present study investigates the effect of transosseous low-intensity pulsed ultrasound (LiUS) on the healing at tendon graft-bone interface, in molecular and histological level. The anterior cruciate ligament (ACL) in ... -
A freestanding proofreading domain is required for protein synthesis quality control in Archaea
Korencic, D.; Ahel, I.; Schelert, J.; Sacher, M.; Ruan, B.; Stathopoulos, C.; Blum, P.; Ibba, M.; Söll, D. (2004)Threonyl-tRNA synthetase (ThrRS) participates in protein synthesis quality control by selectively editing the misacylated species Ser-tRNAThr. In bacteria and eukaryotes the editing function of ThrRS resides in a highly ... -
Genomic analysis of recombinant Sabin clinical isolates
Paximadi, E.; Karakasiliotis, I.; Mamuris, Z.; Stathopoulos, C.; Krikelis, V.; Markoulatos, P. (2006)Recombination in Poliovirus vaccine strains is a very frequent phenomenon. In this report 23 polio/Sabin strains isolated from healthy vaccinees or from VAPP patients after OPV administration, were investigated in order ... -
In Vitro and In Vivo Evaluations of Oxacillin Efficiency against mecA-Positive Oxacillin-Susceptible Staphylococcus aureus
Ikonomidis, A.; Michail, G.; Vasdeki, A.; Labrou, M.; Karavasilis, V.; Stathopoulos, C.; Maniatis, A. N.; Pournaras, S. (2008)Community-type Staphylococcus aureus strains that are positive for mecA and PBP2a but appear phenotypically susceptible to oxacillin are increasingly reported worldwide. Four S. aureus clinical isolates carrying the mecA ... -
Inhibition of human poly(A)-specific ribonuclease (PARN) by purine nucleotides: kinetic analysis
Balatsos, N. A. A.; Anastasakis, D.; Stathopoulos, C. (2009)Poly(A)-specific ribonuclease (PARN) is a cap-interacting and poly(A)-specific 3'-exoribonuclease that efficiently degrades mRNA poly(A) tails. Based on the enzyme's preference for its natural substrates, we examined the ... -
An Integrated In Silico Approach to Design Specific Inhibitors Targeting Human Poly(A)-Specific Ribonuclease
Vlachakis, D.; Pavlopoulou, A.; Tsiliki, G.; Komiotis, D.; Stathopoulos, C.; Balatsos, N. A. A.; Kossida, S. (2012)Poly(A)-specific ribonuclease (PARN) is an exoribonuclease/deadenylase that degrades 3'-end poly(A) tails in almost all eukaryotic organisms. Much of the biochemical and structural information on PARN comes from the human ... -
Intensive care unit dissemination of multiple clones of linezolid-resistant Enterococcus faecalis and Enterococcus faecium
Ntokou, E.; Stathopoulos, C.; Kristo, I.; Dimitroulia, E.; Labrou, M.; Vasdeki, A.; Makris, D.; Zakynthinos, E.; Tsakris, A.; Pournaras, S. (2012)Outbreaks caused by linezolid-resistant (LR) enterococci remain rare. We report the epidemiological and molecular characteristics of the multiclonal dissemination of LR enterococci in the intensive care unit (ICU) of a ... -
Kinetic and in silico analysis of the slow-binding inhibition of human poly(A)-specific ribonuclease (PARN) by novel nucleoside analogues
Balatsos, N.; Vlachakis, D.; Chatzigeorgiou, V.; Manta, S.; Komiotis, D.; Vlassi, M.; Stathopoulos, C. (2012)Poly(A)-specific ribonuclease (PARN) is a 3'-exoribonuclease that efficiently degrades poly(A) tails and regulates, in part, mRNA turnover rates. We have previously reported that adenosine- and cytosine-based glucopyranosyl ... -
Kinetics of inhibition of ribonuclease P activity by peptidyltransferase inhibitors: Effect of antibiotics on RNase P
Kalavrizioti, D.; Vourekas, A.; Tekos, A.; Tsagla, A.; Stathopoulos, C.; Drainas, D. (2003)A cell-free system derived from Dictyostelium discoideum has been used to study the kinetics of inhibition of RNase P by puromycin, amicetin and blasticidin S. Detailed kinetic analysis showed that the type of inhibition ... -
Linezolid Dependence in Staphylococcus epidermidis Bloodstream Isolates
Pournaras, S.; Ntokou, E.; Zarkotou, O.; Ranellou, K.; Themeli-Digalaki, K.; Stathopoulos, C.; Tsakris, A. (2013)We document linezolid dependence among 5 highly linezolid-resistant (LRSE) Staphylococcus epidermidis bloodstream isolates that grew substantially faster at 32 mu g/mL linezolid presence. These isolates carried the mutations ... -
Mitochondrial tRNA mutations - Clinical and functional perturbations
Zifa, E.; Giannouli, S.; Theotokis, P.; Stamatis, O.; Mamuris, Z.; Stathopoulos, C. (2007)During the last decade, there has been a progressive accumulation of reports that connect the identification of specific mitochondrial tRNA gene mutations to severe disorders in human. As a result, mitochondrial tRNA genes ...