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Enhanced expression of miR-21 and miR-150 is a feature of anti-mitochondrial antibody-negative primary biliary cholangitis
dc.creator | Wasik U., Kempinska-Podhorodecka A., Bogdanos D.P., Milkiewicz P., Milkiewicz M. | en |
dc.date.accessioned | 2023-01-31T11:37:22Z | |
dc.date.available | 2023-01-31T11:37:22Z | |
dc.date.issued | 2020 | |
dc.identifier | 10.1186/s10020-019-0130-1 | |
dc.identifier.issn | 10761551 | |
dc.identifier.uri | http://hdl.handle.net/11615/80785 | |
dc.description.abstract | Background & Aims: Anti-mitochondrial-autoantibodies (AMA) remain a hallmark of Primary Biliary Cholangitis (PBC) however approximately 10% of patients test negative for these antibodies. They do not differ in terms of biochemistry or clinical presentation from AMA positive ones. Epigenetics play a key role in immune signalling. Two microRNAs (miRs), namely, miR-21 and miR-150 are known to be involved in liver inflammation and fibrosis. The expression of those two microRNAs and their downstream targets were analyze in the context of AMA-status and the stage of liver fibrosis. Methods: The relative levels of miR-21 and miR-150 and their target genes: cMyb, RAS-guanyl-releasing protein-1(RASGRP1), and DNA-methyltransferase-1(DNMT1) were determined by Real-Time PCR in serum, liver tissue and peripheral blood mononuclear cells (PBMCs) of patients with PBC. Results: Serum expressions of miR-21 and miR-150 were significantly enhanced in AMA-negative patients, and they inversely correlated with disease-specific AMA titers in PBS patients. In PBMCs, an increased expression of miR-21 correlated with decreased levels of RASGRP1 and DNMT1 mRNAs whereas, the level of miR-150 remained comparable to controls; and cMyb mRNA was downregulated. In cirrhotic livers, the level of miR-21 was unchanged while miR-150 expression was increased. Conclusion: This study convincingly report, that AMA-negative PBC is characterized by notable alternations of miR-21 and miR-150 and their downstream targets compared to AMA-positive patients underlining their possible importance in the induction of the disease and its progression to fibrosis. © 2020 The Author(s). | en |
dc.language.iso | en | en |
dc.source | Molecular Medicine | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85078071471&doi=10.1186%2fs10020-019-0130-1&partnerID=40&md5=a4332445a8cd183fff45d8f1b41ea011 | |
dc.subject | microRNA | en |
dc.subject | microRNA 150 | en |
dc.subject | microRNA 21 | en |
dc.subject | mitochondrion antibody | en |
dc.subject | unclassified drug | en |
dc.subject | autoantibody | en |
dc.subject | circulating microRNA | en |
dc.subject | microRNA | en |
dc.subject | MIRN150 microRNA, human | en |
dc.subject | MIRN21 microRNA, human | en |
dc.subject | adult | en |
dc.subject | antibody titer | en |
dc.subject | Article | en |
dc.subject | controlled study | en |
dc.subject | DNMT1 gene | en |
dc.subject | down regulation | en |
dc.subject | female | en |
dc.subject | gene | en |
dc.subject | gene expression | en |
dc.subject | genetic association | en |
dc.subject | human | en |
dc.subject | human cell | en |
dc.subject | human tissue | en |
dc.subject | liver tissue | en |
dc.subject | major clinical study | en |
dc.subject | male | en |
dc.subject | middle aged | en |
dc.subject | peripheral blood mononuclear cell | en |
dc.subject | primary biliary cirrhosis | en |
dc.subject | priority journal | en |
dc.subject | RASGRP1 gene | en |
dc.subject | real time polymerase chain reaction | en |
dc.subject | aged | en |
dc.subject | biliary cirrhosis | en |
dc.subject | biological model | en |
dc.subject | blood | en |
dc.subject | disease predisposition | en |
dc.subject | gene expression regulation | en |
dc.subject | genetic predisposition | en |
dc.subject | genetics | en |
dc.subject | immunology | en |
dc.subject | liver function test | en |
dc.subject | metabolism | en |
dc.subject | mitochondrion | en |
dc.subject | mononuclear cell | en |
dc.subject | risk factor | en |
dc.subject | RNA interference | en |
dc.subject | single nucleotide polymorphism | en |
dc.subject | Aged | en |
dc.subject | Autoantibodies | en |
dc.subject | Circulating MicroRNA | en |
dc.subject | Disease Susceptibility | en |
dc.subject | Female | en |
dc.subject | Gene Expression Regulation | en |
dc.subject | Genetic Predisposition to Disease | en |
dc.subject | Humans | en |
dc.subject | Leukocytes, Mononuclear | en |
dc.subject | Liver Cirrhosis, Biliary | en |
dc.subject | Liver Function Tests | en |
dc.subject | Male | en |
dc.subject | MicroRNAs | en |
dc.subject | Middle Aged | en |
dc.subject | Mitochondria | en |
dc.subject | Models, Biological | en |
dc.subject | Polymorphism, Single Nucleotide | en |
dc.subject | Risk Factors | en |
dc.subject | RNA Interference | en |
dc.subject | BioMed Central | en |
dc.title | Enhanced expression of miR-21 and miR-150 is a feature of anti-mitochondrial antibody-negative primary biliary cholangitis | en |
dc.type | journalArticle | en |
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