Understanding the role of chondrocytes in osteoarthritis: utilizing proteomics
Ημερομηνία
2019Γλώσσα
en
Λέξη-κλειδί
Επιτομή
Introduction: Proteomic analyses have been acknowledged to carry a significant prospective in elucidating the pathogenesis of several diseases, including osteoarthritis (OA). But it has not been an easy road: major technical issues, mainly derived from the complex and rigid nature of the cartilage tissue, had to be faced; an obstacle that led to the development of different approaches. Areas covered: In this review, we categorized the proteomic studies undertaken (proteomic analyses of the cartilage, cartilage explants, cultured chondrocytes, and chondrocytes’ secretome) as part of the different strategies developed in order to overcome tissue and disease-specific challenges. Essentially these approaches aimed at identifying differences in the proteome of healthy vs diseased tissue. Our aim was to point out the novel players that have emerged from these analyses and highlight the associated mechanism(s) suggested to play a role in the pathogenesis of OA. Expert commentary: The identified factors indicate the implication of age-associated mechanisms, such as metabolic deregulation, inflammation, and redox imbalance, in OA onset and/or progression. Taken together these results outline the causal network of the disease and place chondrocytes’ senescence at the center of the emerging aetiopathological atlas. © 2019, © 2019 Informa UK Limited, trading as Taylor & Francis Group.
Collections
Related items
Showing items related by title, author, creator and subject.
-
Milk of Greek sheep and goat breeds; characterization by means of proteomics
Anagnostopoulos A.K., Katsafadou A.I., Pierros V., Kontopodis E., Fthenakis G.C., Arsenos G., Karkabounas S.C., Tzora A., Skoufos I., Tsangaris G.T. (2016)Over the past 30 years there has been a growing interest to unravel the dynamic framework of the milk proteome, and now that available technology is mature enough to enable techniques of protein fractionation and identification, ... -
Estimating the total number of phosphoproteins and phosphorylation sites in eukaryotic proteomes
Vlastaridis P., Kyriakidou P., Chaliotis A., Van de Peer Y., Oliver S.G., Amoutzias G.D. (2017)Background: Phosphorylation is the most frequent post-translational modification made to proteins and may regulate protein activity as either a molecular digital switch or a rheostat. Despite the cornucopia of high-throughput ... -
Differential quantitative proteomics study of experimental Mannheimia haemolytica mastitis in sheep
Katsafadou A.I., Tsangaris G.T., Anagnostopoulos A.K., Billinis C., Barbagianni M.S., Vasileiou N.G.C., Spanos S.A., Mavrogianni V.S., Fthenakis G.C. (2019)Objective was the differential quantitative proteomics study of ovine mastitis induced by Mannheimia haemolytica; clinical, microbiological, cytological and histopathological methods were employed for confirmation and ...