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dc.creatorRavanidis S., Grundler F., de Toledo F.W., Dimitriou E., Tekos F., Skaperda Z., Kouretas D., Doxakis E.en
dc.date.accessioned2023-01-31T09:51:17Z
dc.date.available2023-01-31T09:51:17Z
dc.date.issued2021
dc.identifier10.1016/j.fct.2021.112187
dc.identifier.issn02786915
dc.identifier.urihttp://hdl.handle.net/11615/78473
dc.description.abstractIt is well-established that long-term fasting improves metabolic health, enhances the total antioxidant capacity and increases well-being. MicroRNAs oversee energy homeostasis and metabolic processes and are widely used as circulating biomarkers to identify the metabolic state. This study investigated whether the expression levels of twenty-four metabolism-associated microRNAs are significantly altered following long-term fasting and if these changes correlate with biochemical and redox parameters in the plasma. Thirty-two participants with an average BMI of 28 kg/m2 underwent a 10-day fasting period with a daily intake of 250 kcal under medical supervision. RT-qPCR on plasma small-RNA extracts revealed that the levels of seven microRNAs (miR-19b-3p, miR-22-3p, miR-122-5p, miR-126-3p, miR-142-3p, miR-143-3p, and miR-145-5p) were significantly altered following fasting. Importantly, the expression levels of these microRNAs have been consistently shown to change in the exact opposite direction in pathological states including obesity, diabetes, nonalcoholic steatohepatitis, and cardiovascular disease. Linear regression analyses revealed that among the microRNAs analyzed, anti-inflammatory miR-146-5p expression displayed most correlations with the levels of different biochemical and redox parameters. In silico analysis of fasting-associated microRNAs demonstrated that they target pathways that are highly enriched for intracellular signaling such mTOR, FoxO and autophagy, as well as extracellular matrix (ECM) interactions and cell-senescence. Overall, these data are consistent with a model in which long-term fasting engages homeostatic mechanisms associated with specific microRNAs to improve metabolic signaling regardless of health status. © 2021 Elsevier Ltden
dc.language.isoenen
dc.sourceFood and Chemical Toxicologyen
dc.source.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85104662161&doi=10.1016%2fj.fct.2021.112187&partnerID=40&md5=3cbfbf41159d0134a4c540004ebc9de4
dc.subjectglucoseen
dc.subjectmammalian target of rapamycinen
dc.subjectmicroRNAen
dc.subjectmicroRNA 122 5pen
dc.subjectmicroRNA 126 3pen
dc.subjectmicroRNA 142 3pen
dc.subjectmicroRNA 143 3pen
dc.subjectmicroRNA 145 5pen
dc.subjectmicroRNA 19b 3pen
dc.subjectmicroRNA 22 3pen
dc.subjecttranscription factor FOXOen
dc.subjecttriacylglycerolen
dc.subjectunclassified drugen
dc.subjectcirculating microRNAen
dc.subjectadulten
dc.subjectArticleen
dc.subjectautophagy (cellular)en
dc.subjectbiochemical analysisen
dc.subjectbioinformaticsen
dc.subjectbody massen
dc.subjectcaloric intakeen
dc.subjectcardiovascular diseaseen
dc.subjectcell agingen
dc.subjectcholesterol blood levelen
dc.subjectclinical featureen
dc.subjectcomputer analysisen
dc.subjectcontrolled studyen
dc.subjectcorrelation analysisen
dc.subjectdiabetes mellitusen
dc.subjectdiastolic blood pressureen
dc.subjectfastingen
dc.subjectfemaleen
dc.subjectgene expressionen
dc.subjectgene expression levelen
dc.subjectgene functionen
dc.subjectglucose blood levelen
dc.subjecthealth statusen
dc.subjecthomeostasisen
dc.subjecthumanen
dc.subjecthuman cellen
dc.subjectintracellular signalingen
dc.subjectlaboratory testen
dc.subjectlinear regression analysisen
dc.subjectmaleen
dc.subjectmetabolismen
dc.subjectmTOR signalingen
dc.subjectnonalcoholic steatohepatitisen
dc.subjectobesityen
dc.subjectoxidation reduction reactionen
dc.subjectreverse transcription polymerase chain reactionen
dc.subjectsystolic blood pressureen
dc.subjecttoxicityen
dc.subjecttriacylglycerol blood levelen
dc.subjectwaist circumferenceen
dc.subjectadolescenten
dc.subjectageden
dc.subjectblooden
dc.subjectdiet restrictionen
dc.subjectgene expression profilingen
dc.subjectmiddle ageden
dc.subjectoxidative stressen
dc.subjectphysiologyen
dc.subjectsignal transductionen
dc.subjectyoung adulten
dc.subjectAdolescenten
dc.subjectAdulten
dc.subjectAgeden
dc.subjectCirculating MicroRNAen
dc.subjectFastingen
dc.subjectFemaleen
dc.subjectGene Expression Profilingen
dc.subjectHumansen
dc.subjectMaleen
dc.subjectMiddle Ageden
dc.subjectOxidative Stressen
dc.subjectSignal Transductionen
dc.subjectYoung Adulten
dc.subjectElsevier Ltden
dc.titleFasting-mediated metabolic and toxicity reprogramming impacts circulating microRNA levels in humansen
dc.typejournalArticleen


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