dc.creator | Papatsiros V., Stylianaki I., Papakonstantinou G., Tsekouras N., Bitchava D., Christodoulopoulos G., Papaioannou N. | en |
dc.date.accessioned | 2023-01-31T09:45:00Z | |
dc.date.available | 2023-01-31T09:45:00Z | |
dc.date.issued | 2020 | |
dc.identifier | 10.1089/vim.2020.0087 | |
dc.identifier.issn | 08828245 | |
dc.identifier.uri | http://hdl.handle.net/11615/77870 | |
dc.description.abstract | Genotype 2 strains of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV-2) have been reported sporadically in Europe. Even if, PRRSV-2 reported to be genetically homogenous in Europe due to the introduction of an MLV vaccine strain, independent introductions of PRRSV-2 field strains have been reported. The aim of the present study was to report the complete genome sequence and evaluate the histopathological lesions of a PRRSV-2 strain, isolated for the first time in Greece. During a routine blood sampling in a commercial pig farm, the results revealed positive samples in weaners of 40-60 days for the PRRSV-2, using real-time polymerase chain reaction. The clinical picture was characterized from respiratory symptoms in weaners, as well as coughing and poor performance at finishing stage and less than 3% mortality rate from weaning stage to finishing stage. The use of ORF5 for PRRSV phylogenetic analysis of the isolated PRRSV strain, named "x1544-1 strain", was successfully determined, belonging to the genotype PRRSV-2. Comparison of the obtained sequence revealed nucleotide sequence identity >98% with PRRSV-2 strain VR2332 and other related strains from Denmark and China. The histopathological evaluation revealed diffuse interstitial pneumonia, multifocal interstitial nephritis, while in the lymphoid organs, follicular and paracortical hyperplasia, coexisting with necrosis and depletion of germ cells were detected. The results of current study undersign the importance for veterinary practitioners to have up-to-date access to phylogenetic data linked to phenotypic information to follow-up the control and prevention strategies against PRRSV. © Copyright 2020, Mary Ann Liebert, Inc., publishers 2020. | en |
dc.language.iso | en | en |
dc.source | Viral Immunology | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85092473606&doi=10.1089%2fvim.2020.0087&partnerID=40&md5=8904dc12cc332ad4db74137b5ebaad21 | |
dc.subject | animal tissue | en |
dc.subject | Article | en |
dc.subject | blood sampling | en |
dc.subject | chilblain | en |
dc.subject | China | en |
dc.subject | coughing | en |
dc.subject | Denmark | en |
dc.subject | follow up | en |
dc.subject | genotype | en |
dc.subject | germ cell | en |
dc.subject | Greece | en |
dc.subject | histopathology | en |
dc.subject | hyperplasia | en |
dc.subject | interstitial nephritis | en |
dc.subject | interstitial pneumonia | en |
dc.subject | lymphoid organ | en |
dc.subject | mortality rate | en |
dc.subject | necrosis | en |
dc.subject | nonhuman | en |
dc.subject | nucleotide sequence | en |
dc.subject | open reading frame | en |
dc.subject | phylogeny | en |
dc.subject | pig | en |
dc.subject | polymerase chain reaction | en |
dc.subject | Porcine reproductive and respiratory syndrome virus | en |
dc.subject | Porcine reproductive and respiratory syndrome virus 2 | en |
dc.subject | real time polymerase chain reaction | en |
dc.subject | virus isolation | en |
dc.subject | weaner | en |
dc.subject | weaning | en |
dc.subject | agricultural land | en |
dc.subject | animal | en |
dc.subject | classification | en |
dc.subject | female | en |
dc.subject | genetic variation | en |
dc.subject | genetics | en |
dc.subject | genotype | en |
dc.subject | Greece | en |
dc.subject | histology | en |
dc.subject | isolation and purification | en |
dc.subject | lung | en |
dc.subject | lymphoid tissue | en |
dc.subject | pathogenicity | en |
dc.subject | pathology | en |
dc.subject | pneumonia | en |
dc.subject | porcine reproductive and respiratory syndrome | en |
dc.subject | Porcine reproductive and respiratory syndrome virus | en |
dc.subject | veterinary medicine | en |
dc.subject | virology | en |
dc.subject | virus genome | en |
dc.subject | virus RNA | en |
dc.subject | Animals | en |
dc.subject | China | en |
dc.subject | Denmark | en |
dc.subject | Farms | en |
dc.subject | Female | en |
dc.subject | Genetic Variation | en |
dc.subject | Genome, Viral | en |
dc.subject | Genotype | en |
dc.subject | Greece | en |
dc.subject | Histological Techniques | en |
dc.subject | Lung | en |
dc.subject | Lymphoid Tissue | en |
dc.subject | Phylogeny | en |
dc.subject | Pneumonia | en |
dc.subject | Porcine Reproductive and Respiratory Syndrome | en |
dc.subject | Porcine respiratory and reproductive syndrome virus | en |
dc.subject | RNA, Viral | en |
dc.subject | Swine | en |
dc.subject | Mary Ann Liebert Inc. | en |
dc.title | Histopathological Lesions Accompanied with First-Time Isolation of a PRRSV-2 Strain in Greece | en |
dc.type | journalArticle | en |