dc.creator | Kakavas K.V. | en |
dc.date.accessioned | 2023-01-31T08:29:13Z | |
dc.date.available | 2023-01-31T08:29:13Z | |
dc.date.issued | 2021 | |
dc.identifier | 10.1007/s11033-021-06349-2 | |
dc.identifier.issn | 03014851 | |
dc.identifier.uri | http://hdl.handle.net/11615/74146 | |
dc.description.abstract | PCR Single-Strand Conformation Polymorphism is a method used to identify and detect mutations and is now well known for its many applications on living beings. This paper will discuss the experimental details, limitations and sensitivity of the PCR Single-Strand Conformation Polymorphism method in relation to all existing literature available to us until today. Genomic DNA extraction, PCR amplification and Single-Strand Conformation Polymorphism conditions (concentration of polyacrylamide slab gel electrophoresis, dissociation treatment of double- stranded DNA) and comparison with PCR Restriction Fragment Length Polymorphism are presented. Since its discovery in 1989, there have been many variations, innovations, and modifications of the method, which makes it very easy, safe, fast and for this reason widely applied in clinical diagnostic, forensic medicine, biochemical, veterinary, microbiological, food and environmental laboratories. One of the possible applications of the method is the diagnosis and identification of mutations in new strains of coronaviruses, because science needs more tools to tackle the problem of this pandemic. The PCR Single-Strand Conformation Polymorphism method can be applied in many cases provided that control samples are available and the required conditions of the method are achieved. © 2021, The Author(s), under exclusive licence to Springer Nature B.V. | en |
dc.language.iso | en | en |
dc.source | Molecular Biology Reports | en |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85105185383&doi=10.1007%2fs11033-021-06349-2&partnerID=40&md5=4c85f1363d58d42379fbbb395021be91 | |
dc.subject | double stranded DNA | en |
dc.subject | genomic DNA | en |
dc.subject | Amphibia | en |
dc.subject | bacterial flora | en |
dc.subject | biochemistry | en |
dc.subject | dissociation | en |
dc.subject | DNA extraction | en |
dc.subject | forensic medicine | en |
dc.subject | fungal flora | en |
dc.subject | gel electrophoresis | en |
dc.subject | gene mutation | en |
dc.subject | genetic variation | en |
dc.subject | human | en |
dc.subject | nonhuman | en |
dc.subject | Phytophthora | en |
dc.subject | polymerase chain reaction restriction fragment length polymorphism | en |
dc.subject | Review | en |
dc.subject | sensitivity analysis | en |
dc.subject | Severe acute respiratory syndrome coronavirus 2 | en |
dc.subject | shrimp | en |
dc.subject | single strand conformation polymorphism | en |
dc.subject | veterinary medicine | en |
dc.subject | virus strain | en |
dc.subject | animal | en |
dc.subject | classification | en |
dc.subject | Coronavirinae | en |
dc.subject | genetics | en |
dc.subject | isolation and purification | en |
dc.subject | molecular pathology | en |
dc.subject | molecular typing | en |
dc.subject | polymerase chain reaction | en |
dc.subject | procedures | en |
dc.subject | restriction fragment length polymorphism | en |
dc.subject | sequence analysis | en |
dc.subject | Animals | en |
dc.subject | Coronavirus | en |
dc.subject | Humans | en |
dc.subject | Molecular Typing | en |
dc.subject | Pathology, Molecular | en |
dc.subject | Polymerase Chain Reaction | en |
dc.subject | Polymorphism, Restriction Fragment Length | en |
dc.subject | Polymorphism, Single-Stranded Conformational | en |
dc.subject | Sequence Analysis | en |
dc.subject | Springer Science and Business Media B.V. | en |
dc.title | Sensitivity and applications of the PCR Single-Strand Conformation Polymorphism method | en |
dc.type | other | en |