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AtHESPERIN: A novel regulator of circadian rhythms with poly(A)-degrading activity in plants

dc.creatorDelis C., Krokida A., Tomatsidou A., Tsikou D., Beta R.A.A., Tsioumpekou M., Moustaka J., Stravodimos G., Leonidas D.D., Balatsos N.A.A., Papadopoulou K.K.en
dc.date.accessioned2023-01-31T07:53:11Z
dc.date.available2023-01-31T07:53:11Z
dc.date.issued2016
dc.identifier10.1080/15476286.2015.1119363
dc.identifier.issn15476286
dc.identifier.urihttp://hdl.handle.net/11615/73193
dc.description.abstractWe report the identification and characterization of a novel gene, AtHesperin (AtHESP) that codes for a deadenylase in Arabidopsis thaliana. The gene is under circadian clock-gene regulation and has similarity to the mammalian Nocturnin. AtHESP can efficiently degrade poly(A) substrates exhibiting allosteric kinetics. Size exclusion chromatography and native electrophoresis coupled with kinetic analysis support that the native enzyme is oligomeric with at least 3 binding sites. Knockdown and overexpression of AtHESP in plant lines affects the expression and rhythmicity of the clock core oscillator genes TOC1 and CCA1. This study demonstrates an evolutionary conserved poly(A)-degrading activity in plants and suggests deadenylation as a mechanism involved in the regulation of the circadian clock. A role of AtHESP in stress response in plants is also depicted. © 2016 Taylor & Francis Group, LLC.en
dc.language.isoenen
dc.sourceRNA Biologyen
dc.source.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84964047200&doi=10.1080%2f15476286.2015.1119363&partnerID=40&md5=1ae8583c3e464c3911dea8e85764faae
dc.subjectdeadenylaseen
dc.subjectenzymeen
dc.subjectpolyadenylic aciden
dc.subjectunclassified drugen
dc.subjectArabidopsis proteinen
dc.subjectCCA1 protein, Arabidopsisen
dc.subjectpolyadenylic aciden
dc.subjectTOC1 protein, Arabidopsisen
dc.subjecttranscription factoren
dc.subjectallosterismen
dc.subjectamino acid sequenceen
dc.subjectArabidopsis thalianaen
dc.subjectArticleen
dc.subjectAtHESP geneen
dc.subjectbinding siteen
dc.subjectCCA1 geneen
dc.subjectchemical reactionen
dc.subjectcircadian rhythmen
dc.subjectcontrolled studyen
dc.subjectdeadenylationen
dc.subjectelectrophoresisen
dc.subjectgene identificationen
dc.subjectgene overexpressionen
dc.subjectgene silencingen
dc.subjectgenetic analysisen
dc.subjectgenetic codeen
dc.subjectkineticsen
dc.subjectnonhumanen
dc.subjectoligomerizationen
dc.subjectplant geneen
dc.subjectRNA degradationen
dc.subjectsize exclusion chromatographyen
dc.subjectTOC1 geneen
dc.subjectArabidopsisen
dc.subjectchemistryen
dc.subjectconserved sequenceen
dc.subjectgene expression regulationen
dc.subjectgeneticsen
dc.subjectgrowth, development and agingen
dc.subjectmetabolismen
dc.subjectmolecular cloningen
dc.subjectoxidative stressen
dc.subjectprotein multimerizationen
dc.subjectAmino Acid Sequenceen
dc.subjectArabidopsisen
dc.subjectArabidopsis Proteinsen
dc.subjectBinding Sitesen
dc.subjectCircadian Rhythmen
dc.subjectCloning, Molecularen
dc.subjectConserved Sequenceen
dc.subjectGene Expression Regulation, Planten
dc.subjectOxidative Stressen
dc.subjectPoly Aen
dc.subjectProtein Multimerizationen
dc.subjectTranscription Factorsen
dc.subjectTaylor and Francis Inc.en
dc.titleAtHESPERIN: A novel regulator of circadian rhythms with poly(A)-degrading activity in plantsen
dc.typejournalArticleen


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