Proteins from fish eggs that protect DNA from acid precipitation and inhibit DNA synthesis

dc.creator	Tsamis, V.	en
dc.creator	Mamuris, Z.	en
dc.creator	Panagiotaki, P.	en
dc.creator	Kouretas, D.	en
dc.date.accessioned	2015-11-23T10:50:48Z
dc.date.available	2015-11-23T10:50:48Z
dc.date.issued	2001
dc.identifier	10.1016/s1532-0456(01)00215-0
dc.identifier.issn	1532-0456
dc.identifier.uri	http://hdl.handle.net/11615/33791
dc.description.abstract	We partially characterized proteins that inhibit DNA acid precipitation from various fish eggs (Sparus aurata, Dicentrarchus labrax, Mugil cephalus and Zeus faber). The active proteins were purified by acetone fractionation. The activity was found to be heat resistant. Of bivalent cations tested only Co2+ and Cu2+ exerted a profound promoting effect in the activity from all fish. The protein fraction from Sparus aurata inhibited DNA synthesis in PCR performed by different DNA polymerases. The possible role of DNA protective proteins in fish egg physiology is discussed. (C) 2001 Elsevier Science Inc. All rights reserved.	en
dc.source.uri	<Go to ISI>://WOS:000170472400008
dc.subject	fish eggs	en
dc.subject	fish egg protein	en
dc.subject	DNA synthesis	en
dc.subject	DNA acid precipitation	en
dc.subject	fetoprotein	en
dc.subject	PCR	en
dc.subject	POLY(U)	en
dc.subject	SERUM	en
dc.subject	RNA	en
dc.subject	Biochemistry & Molecular Biology	en
dc.subject	Endocrinology & Metabolism	en
dc.subject	Toxicology	en
dc.subject	Zoology	en
dc.title	Proteins from fish eggs that protect DNA from acid precipitation and inhibit DNA synthesis	en
dc.type	journalArticle	en

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