dc.creator | Satra, M. | en |
dc.creator | Tsougos, I. | en |
dc.creator | Papanikolaou, V. | en |
dc.creator | Theodorou, K. | en |
dc.creator | Kappas, C. | en |
dc.creator | Tsezou, A. | en |
dc.date.accessioned | 2015-11-23T10:47:03Z | |
dc.date.available | 2015-11-23T10:47:03Z | |
dc.date.issued | 2006 | |
dc.identifier | 10.1080/09553000600800090 | |
dc.identifier.issn | 0955-3002 | |
dc.identifier.uri | http://hdl.handle.net/11615/32908 | |
dc.description.abstract | Purpose: To quantify and correlate human telomerase reverse transcriptase (hTERT) mRNA expression with telomerase activity (TA) after ionizing irradiation of HeLa cells. Materials and methods: TA and hTERT mRNA expression were evaluated, at 24-h intervals, in HeLa cells cultured for up to 144 h, before and after treatment with increasing doses of 6 MV photon ionizing radiation (5-20 Gy), using the telomeric repeat amplification protocol (TRAP) assay and real-time reverse transcriptase polymerase chain reaction (RT-PCR), respectively. Cell viability was determined using the 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. A prototype phantom was constructed for accurate irradiation of HeLa cells. Results: Treated cells showed a decrease in viability with increasing radiation dose, and a correlation was observed with post-treatment period. TA and hTERT mRNA expression of HeLa cells increased for the first 24 h after irradiation. The maximal increases were approximately two times the un-irradiated cell levels at 24 h post-irradiation, followed by a decrease and a return to the control levels 72 h post-irradiation. The time-course of telomerase activation after 24 h, differed among radiation doses. A dose-dependent G2/M arrest was observed 24 h post-irradiation, along with an increase in polyploidy 48 h post-irradiation and afterwards. Conclusion: A correlation between TA and hTERT mRNA expression and a radiation induced cell cycle dependent modification of hTERT mRNA expression was established for the first 24 h post-irradiation. | en |
dc.source | International Journal of Radiation Biology | en |
dc.source.uri | <Go to ISI>://WOS:000239146400003 | |
dc.subject | telomerase activity | en |
dc.subject | hTERT | en |
dc.subject | ionizing radiation | en |
dc.subject | HeLa cells | en |
dc.subject | LETHAL MUTATIONS | en |
dc.subject | CANCER | en |
dc.subject | IRRADIATION | en |
dc.subject | GENE | en |
dc.subject | RADIOCURABILITY | en |
dc.subject | RADIOTHERAPY | en |
dc.subject | INSTABILITY | en |
dc.subject | PROGENY | en |
dc.subject | TUMORS | en |
dc.subject | PCR | en |
dc.subject | Biology | en |
dc.subject | Nuclear Science & Technology | en |
dc.subject | Radiology, Nuclear Medicine & | en |
dc.subject | Medical Imaging | en |
dc.title | Correlation between radiation-induced telomerase activity and human telomerase reverse transcriptase mRNA expression in HeLa cells | en |
dc.type | journalArticle | en |