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Μελέτη του γενετικού μηχανισμού αντοχής στις καρβαπενέμες σε κλινικά στελέχη Pseudomonas aeruginosa
dc.contributor.advisor | Μόσιαλος, Δημήτρης | el |
dc.creator | Σακελλάρης, Βίκτωρ | el |
dc.date.accessioned | 2015-01-05T21:42:40Z | |
dc.date.available | 2015-01-05T21:42:40Z | |
dc.date.issued | 2010 | |
dc.identifier.other | 8172 | |
dc.identifier.uri | http://hdl.handle.net/11615/305 | en |
dc.description.abstract | Pseudomonas aeruginosa is one of the most common gram-negative hospitalacquired pathogens. This organism shows a remarkable resistance to antibiotics, mostly because of acquisition of resistance genes e.g., genes for beta-lactamases, combined with over-expression of efflux pumps and/or decreased expression of porins. The most common beta-lactamases are IMP and VIM type MBL, as well as OXA-ESBLS and give to the bacteria high resistance to b-lactam antibiotics, especially carbapenems. The genes for VIM and IMP enzymes, like those for OXA-ESBLs, are transferable as most are found as gene cassettes located primarily on integrons, sometimes within transposons or on plasmids, a condition that provides a wide potential for expression and dissemination in gram-negative pathogens. Since the first finding of IMP] metallo-B-lactamase in a clinical isolate of Pseudomonas aeruginosa in Japan in 1988, IMP-type enzymes are spread worldwide, increasing in two decades the proportion of P. aeruginosa isolates demonstrating resistance to imipenem, due to IMPmbls? from 13% to 20%. Currently have indentified 26 carbapenem-hydrolyzing enzymes of the IMP-type. In this study we present the discovery of a new IMP type MBL from 6 P. aeruginosa clinical strains. This novel IMP-type MBL is highly divergent from IMPi, possessing 44 different amino acids, whereas the most divergent till now IMP-type MBL, IMP12, has only 36 different amino acids from IMP]. This new IMP-MBLs was found in a class 1 integron next to ΟΧʼ35 gene. The next objective will be the biochemical characterization of this new IMP metallo-fl-lactamase. | en |
dc.language.iso | el | en |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International | en |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | en |
dc.subject.other | ΓΟΝΙΔΙΑ -- ΑΝΑΛΥΣΗ | el |
dc.subject.other | ΑΝΤΙΒΙΟΤΙΚΑ | el |
dc.subject.other | GRAM ΑΡΝΗΤΙΚΑ ΒΑΚΤΗΡΙΑ | el |
dc.title | Μελέτη του γενετικού μηχανισμού αντοχής στις καρβαπενέμες σε κλινικά στελέχη Pseudomonas aeruginosa | el |
dc.type | bachelorThesis | en |
heal.recordProvider | Πανεπιστήμιο Θεσσαλίας - Βιβλιοθήκη και Κέντρο Πληροφόρησης | el |
heal.academicPublisher | Πανεπιστήμιο Θεσσαλίας. Σχολή Επιστημών Υγείας. Τμήμα Βιοχημείας και Βιοτεχνολογίας. | el |
heal.academicPublisherID | uth | en |
heal.fullTextAvailability | true | en |
dc.rights.accessRights | free | en |