dc.creator | Makris, D. | en |
dc.creator | Lazarou, S. | en |
dc.creator | Alexandrakis, M. | en |
dc.creator | Kourelis, T. V. | en |
dc.creator | Tzanakis, N. | en |
dc.creator | Kyriakou, D. | en |
dc.creator | Gourgoulianis, K. I. | en |
dc.date.accessioned | 2015-11-23T10:38:26Z | |
dc.date.available | 2015-11-23T10:38:26Z | |
dc.date.issued | 2008 | |
dc.identifier | 10.1378/chest.07-2626 | |
dc.identifier.issn | 123692 | |
dc.identifier.uri | http://hdl.handle.net/11615/30520 | |
dc.description.abstract | Background: T lymphocytes and especially the subpopulations of CD8+ cells are believed to have a key role in COPD pathophysiology, but there are only few data regarding the role of these cells in COPD exacerbation. Aim: We aimed to study prospectively changes of CD8+ T-lymphocyte subpopulations in the sputum of COPD patients at the onset of mild exacerbations and at a stable condition in order to provide further insight in the pathophysiology of the disease. Methods: Induced-sputum samples were collected from 24 COPD patients with median age of 52 years (interquartile range [IQR], 44 to 58 years) and FEV1 percentage of predicted of 78.05% (IQR, 75.8 to 80.1%) at the onset of mild exacerbations not requiring hospitalization and when stable. Inflammatory cells and T-lymphocyte subpopulations (CD4+, CD8+, and cells producing interferon [IFN]-γ or interleukin [IL]-4) were measured using flow cytometry and immunocytochemical methods. Results: A significant increase in sputum CD8+ T lymphocytes (p < 0.0001) and significant decreases in CD4+ T lymphocytes as well as in CD4+/CD8+ (p = 0.0001) and CD8+IFN-γ+/CD8+IL-4+ (p = 0.001), CD4+IFN-γ+/CD4+IL-4+ (p = 0.0003) sputum cells ratios were found decreased at the onset of exacerbations compared to stable condition. The changes in T-lymphocyte subpopulations were not associated with smoking history, demographic characteristics, or disease severity. Conclusion: The findings of the present study suggest that CD8+ lymphocytes are increased and potentially polarized toward a Tc2 profile in the airways of COPD patients at the onset of COPD exacerbations with respect to stable condition. The clinical impact of the observed phenomenon requires further investigation. Copyright © 2008 by American College of Chest Physicians. | en |
dc.source.uri | http://www.scopus.com/inward/record.url?eid=2-s2.0-51649104325&partnerID=40&md5=631d805a1507e1caaa2b99ebf5c80abe | |
dc.subject | COPD | en |
dc.subject | Exacerbations | en |
dc.subject | Lymphocytes | en |
dc.subject | gamma interferon | en |
dc.subject | interleukin 4 | en |
dc.subject | adult | en |
dc.subject | article | en |
dc.subject | CD4+ T lymphocyte | en |
dc.subject | CD8+ T lymphocyte | en |
dc.subject | cell subpopulation | en |
dc.subject | chronic obstructive lung disease | en |
dc.subject | clinical article | en |
dc.subject | disease exacerbation | en |
dc.subject | disease severity | en |
dc.subject | eosinophil count | en |
dc.subject | female | en |
dc.subject | flow cytometry | en |
dc.subject | forced expiratory volume | en |
dc.subject | hospitalization | en |
dc.subject | human | en |
dc.subject | human cell | en |
dc.subject | immune response | en |
dc.subject | immunocytochemistry | en |
dc.subject | inflammatory cell | en |
dc.subject | lymphocyte count | en |
dc.subject | macrophage | en |
dc.subject | male | en |
dc.subject | neutrophil count | en |
dc.subject | pathophysiology | en |
dc.subject | priority journal | en |
dc.subject | smoking | en |
dc.subject | sputum | en |
dc.subject | sputum analysis | en |
dc.subject | CD4-CD8 Ratio | en |
dc.subject | CD4-Positive T-Lymphocytes | en |
dc.subject | CD8-Positive T-Lymphocytes | en |
dc.subject | Humans | en |
dc.subject | Interferon Type II | en |
dc.subject | Interleukin-4 | en |
dc.subject | Lymphocyte Subsets | en |
dc.subject | Middle Aged | en |
dc.subject | Prospective Studies | en |
dc.subject | Pulmonary Disease, Chronic Obstructive | en |
dc.subject | T-Lymphocytes, Cytotoxic | en |
dc.title | Tc2 response at the onset of COPD exacerbations | en |
dc.type | journalArticle | en |