Mostra i principali dati dell'item
Correlation of promoter hypermethylation in hTERT, DAPK and MGMT genes with cervical oncogenesis progression
dc.creator | Iliopoulos, D. | en |
dc.creator | Oikonomou, P. | en |
dc.creator | Messinis, I. | en |
dc.creator | Tsezou, A. | en |
dc.date.accessioned | 2015-11-23T10:30:22Z | |
dc.date.available | 2015-11-23T10:30:22Z | |
dc.date.issued | 2009 | |
dc.identifier | 10.3892/or_00000425 | |
dc.identifier.issn | 1021-335X | |
dc.identifier.uri | http://hdl.handle.net/11615/28578 | |
dc.description.abstract | DNA hypermethylation occurs during the multi-step process of cervical carcinogenesis. We investigated whether the methylation status in the promoter region of a potential oncogene, the human telomerase reverse transcriptase (hTERT), and the tumor suppressor genes death-associated protein kinase (DAPK) and O(6)-methylguanine DNA methyltransferase (MGMT), were able to distinguish the early from late stages of cervical oncogenesis. The methylation status in the promoter of these genes was analyzed using real-time MethyLight analysis in 115 cervical specimens, including normal, premalignant [atypical squamous epithelial cells (ASCUS), low-grade squamous intraepithelial lesions (LGSIL), high-grade squamous intraepithelial lesions (HGSIL)] and cancer specimens. Clinicopathological parameters (cytology, histology, grade, stage) were compared to the levels of pro-moter hypermethylation. We found that hTERT, MGMT and DAPK hypermethylation levels were increased during cervical oncogenesis progression. hTERT promoter hypermethylation was able to distinguish normal from cancer (p=0.008), normal from premalignant (p=0.036), its well as premalignant from cervical cancer cases (p=0.003). A significant association was also observed between all three genes and the grade of cervical cancer, with hTERT showing a better association (p<0.0001). Our data suggest that the combination of hTERT, MGMT, DAPK promoter hypermethylation could have a potential function as molecular biomarker of cervical oncogenesis progression. | en |
dc.source.uri | <Go to ISI>://WOS:000267259100028 | |
dc.subject | DNA methylation | en |
dc.subject | telomerase | en |
dc.subject | methylguanine DNA methyltransferase | en |
dc.subject | death-associated protein kinase | en |
dc.subject | human telomerase reverse transcriptase | en |
dc.subject | SQUAMOUS-CELL CARCINOMA | en |
dc.subject | NEOPLASIA GRADE-III | en |
dc.subject | HUMAN-PAPILLOMAVIRUS | en |
dc.subject | CATALYTIC SUBUNIT | en |
dc.subject | HUMAN TELOMERASE | en |
dc.subject | DNA METHYLATION | en |
dc.subject | MICROSATELLITE | en |
dc.subject | INSTABILITY | en |
dc.subject | CANCER PATIENTS | en |
dc.subject | EXPRESSION | en |
dc.subject | TUMORS | en |
dc.subject | Oncology | en |
dc.title | Correlation of promoter hypermethylation in hTERT, DAPK and MGMT genes with cervical oncogenesis progression | en |
dc.type | journalArticle | en |
Files in questo item
Files | Dimensione | Formato | Mostra |
---|---|---|---|
Nessun files in questo item. |