| dc.description.abstract | Despite its extensive use for evaluation of spermatogenesis and assisted reproduction the safety and consequences of fine (FNA) and large needle aspiration (LNA) to the testicular parenchyma and its normal function have not been established This study was performed in order to accurately assess, by serial in vitro ultrasonographic, bacteriologic, gross anatomic and histological examinations the type and extent of the effect of FNA or LNA on the dog's testis Twenty three sexually mature 1 to 2 years old, healthy laboratory Beagles were randomly assigned to 2 groups (1) 5 dogs without testicular aspiration (control group) and (2) 18 dogs in which one of their testes was aspirated using a 23 G butterfly needle and the other using a 190 butterfly needle (experimental group) Two dogs at a time were castrated 10 minutes 60 minutes, 2, 14 29, 63, 76 90 or 180 days post-aspiration The control group was also castrated 2 29 63 90 or 180 days after the beginning of the experiment Following castration, in vitro ultrasonographic, gross anatomic, cytological examinations of epididymal sperm, bacteriologic and histological examinations of the testes were performed Following testicular FNA and LNA bacteriologic, gross anatomic, histologic, epididymal sperm findings and the in vitro ultrasonographic appearance of the testis were normal, except of intratesticular haemorrhage detected the first days post aspiration, and degeneration of less than 1 5% of the seminiferous tubules Within the parameters of this experiment, testicular FNA and LNA have no ill effect on the canine testis and therefore both FNA and LNA should be considered safe (C) 2010 Elsevier Inc All rights reserved | en |
