dc.creator | Dimopoulou, M. | en |
dc.creator | Anifandis, G. | en |
dc.creator | Messini, C. I. | en |
dc.creator | Dafopoulos, K. | en |
dc.creator | Kouris, S. | en |
dc.creator | Sotiriou, S. | en |
dc.creator | Satra, M. | en |
dc.creator | Vamvakopoulos, N. | en |
dc.creator | Messinis, I. E. | en |
dc.date.accessioned | 2015-11-23T10:25:44Z | |
dc.date.available | 2015-11-23T10:25:44Z | |
dc.date.issued | 2014 | |
dc.identifier | 10.1155/2014/289306 | |
dc.identifier.issn | 2314-6133 | |
dc.identifier.uri | http://hdl.handle.net/11615/27120 | |
dc.description.abstract | The present prospective study examined the follicular fluid oocyte/cumulus-free DNA concentrations (ff o/c-free DNA) during ovarian stimulation and the possible association between ff o/c-free DNA and embryological results such as embryo quality and pregnancy rate. Eighty-three women undergoing IV/ICSI-ET treatments were prospectively included in this study. ff o/c-free DNA was determined by conventional quantitative real time PCR-Sybr green detection approach. The 83 ff samples were categorized in two groups: group 1 (n = 62) with cumulus oocytes complexes (CoCs) >= 2 and group 2 (n = 21) with CoCs = 1. Group 1 revealed significant higher embryo quality in terms of mean score of embryo transfer (MSET), but lower ff o/c-free DNA concentrations compared to group 2. The two groups showed comparable pregnancy rates (positive hCG and clinical pregnancy). The higher the ff o/c-free DNA concentration, the lower the number of produced oocytes. ff o/c-free DNA did not seem to have any direct role in the IVF outcome. Further research is required to clarify whether ff o/c-free DNA is a biomolecular marker of embryo quality and IVF outcome. | en |
dc.source.uri | <Go to ISI>://WOS:000338103600001 | |
dc.subject | CELL-FREE DNA | en |
dc.subject | PLASMA | en |
dc.subject | PREGNANCY | en |
dc.subject | DISEASE | en |
dc.subject | CANCER | en |
dc.subject | Biotechnology & Applied Microbiology | en |
dc.subject | Medicine, Research & Experimental | en |
dc.title | Follicular Fluid Oocyte/Cumulus-Free DNA Concentrations as a Potential Biomolecular Marker of Embryo Quality and IVF Outcome | en |
dc.type | journalArticle | en |