dc.creator | Chaidos, A. I. | en |
dc.creator | Bai, M. C. | en |
dc.creator | Kamina, S. A. | en |
dc.creator | Kanavaros, P. E. | en |
dc.creator | Agnantis, N. J. | en |
dc.creator | Bourantas, K. L. | en |
dc.date.accessioned | 2015-11-23T10:24:24Z | |
dc.date.available | 2015-11-23T10:24:24Z | |
dc.date.issued | 2002 | |
dc.identifier | 10.1034/j.1600-0609.2002.01634.x | |
dc.identifier.issn | 0902-4441 | |
dc.identifier.uri | http://hdl.handle.net/11615/26543 | |
dc.description.abstract | We evaluated the in vivo incidence of apoptosis and cell proliferation in multiple myeloma (MM) and investigated the correlation of both cellular events with histological tumour stage and grade, bcl-2 protein expression, serum IL-6 and sIL-6R. Material and methods: The TUNEL method was used to assess apoptosis and immunohistochemistry to assess the expression of proliferating cell nuclear antigen (PCNA) and bcl-2 protein in 30 bone marrow biopsy specimens. The apoptotic index (AI) and proliferative index (PI) were defined as the percentage of TUNEL and PCNA positive plasma cells, respectively. Results: The mean AI was 0.162% and the mean PI 27.44%. A positive correlation between AI and PI was found (r=0.44, P=0.017). PI was also correlated with tumour grade (P=0.015). The mean bcl-2 protein expression was 70% and did not correlate with AI or PI, but was higher in specimens taken at first diagnosis than in specimens taken after response to treatment (P=0.035). The mean serum IL-6 and sIL-6R values were 9.43 pg mL(-1) and 47.27 ng mL(-1), respectively. These parameters did not correlate with AI or PI. Conclusions: The results indicate that MM might be among the malignancies with very low incidence of apoptosis. Proliferative activity increased in parallel with tumour histological grade. A positive correlation between apoptosis and proliferation was observed, but the incidence of these two cellular events seems not to be related to the bcl-2 protein expression and the serum levels of IL-6 and sIL-6R. | en |
dc.source | European Journal of Haematology | en |
dc.source.uri | <Go to ISI>://WOS:000178481400003 | |
dc.subject | multiple myeloma | en |
dc.subject | apoptosis | en |
dc.subject | cell proliferation | en |
dc.subject | bcl-2 | en |
dc.subject | interleukin-6 | en |
dc.subject | NON-HODGKINS-LYMPHOMAS | en |
dc.subject | BONE-MARROW | en |
dc.subject | PLASMA-CELLS | en |
dc.subject | GROWTH | en |
dc.subject | DEATH | en |
dc.subject | CLASSIFICATION | en |
dc.subject | KI67 | en |
dc.subject | P53 | en |
dc.subject | Hematology | en |
dc.title | Incidence of apoptosis and cell proliferation in multiple myeloma - Correlation with bcl-2 protein expression and serum levels of interleukin-6 (IL-6) and soluble IL-6 receptor | en |
dc.type | journalArticle | en |